CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



721 



0.8% Xahrstoff-Heyden in the prepa- 

 ration of medium 2239. 

 (e) Levine and Carpenter studied the 

 liquefaction of gelatin on a medium 

 prepared as follows: 



(1) Add 1.0 g. Difco peptone, 35.0 g. 

 Difco gelatin to 1000.0 cc. distilled 

 water and heat, at 60-65°C. until all 

 material is in solution. 



(2) Heat in double boiler for 15.0 

 minutes. 



(3) Adjust to pH = 8.0. 



(4) Make up volume. 



(5) Heat 15.0 more minutes. 



(6) Filter thru cotton flannel. 



(7) Distribute in 25.0 cc. quantities. 



(8) Sterilize at 15.0 pounds for 15.0 

 minutes. 



References: Jacobi (1888 p. 539), Matzu- 

 schita (1902 p. 286), Lieske (1919 p. 417), 

 Weiss (1920 p. 24), Levine and Carpenter 

 (1923 p. 298). 



2241. Metchnikoff's Peptone Gelatin 

 (Thoinot and Masselin) 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 10.0 g. 



4. Gelatin 20.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Enrichment of Bacillus of Asiatic 

 cholera. Sergent cultivated B. zopfi on 

 a similar medium. 



Variants : 



(a) Wherry gave the following method of 

 preparation: 



(1) Dissolve 200.0 g. Gold Label gela- 

 tin, 10.0 g. Witte's peptone and 

 5.0 g. NaCl in 1000.0 cc. of water 

 by boiling. 



(2) Divide in two 500.0 cc. portions. 



(3) Adjust one portion to +1 reaction 

 to phenolphthalein with normal 

 NaOH. To the other portion add 

 an equal volume of normal Na2C03. 



(4) Cool to 40°C., add whites of three 

 eggs to each. 



(5) Boil for 3 minutes. 



(6) Filter thru cotton and distribute. 



(7) Sterilize in Arnold for 20 minutes 

 on each of 3 successive days. 



(8) Final reaction of NaOH portion 

 -M.7. 



Final reaction of Na2C03 portion 

 -M.S. 



(b) Sergent cultivated Bact. zopfi on a 

 medium prepared by dissolving 5.0 g. 

 NaCl, 10.0 g. peptone and 80.0 g. 

 gelatin in 1000.0 cc. distilled water. 



(c) Roux and Rochaix dissolved 10.0 g. 

 peptone, 0.5 g. NaCl and 20.0 g. 

 gelatin in 1000.0 cc. water and made 

 the reaction slightly alkaline (indi- 

 cator not specified). 



(d) Besson prepared the medium as 

 follows: 



(1) Dissolve 10.0 g. Chapoteaut's pep- 

 tone, 5.0 g. NaCl, and 20.0 g. 

 gelatin (extra white) in 1000.0 cc. 

 water. 



(2) Make slightly alkaline by the addi- 

 tion of soda solution. 



(3) Heat 5 minutes at 115°C. 



(4) Filter thru paper. 



(5) Distribute as desired. 



(6) Sterilize at 110 to 115°C. 

 References: Thoinot and Masselin (1902 



p. 392), Wherry (1905 p. 320), Sergent 

 (1906 p. 1015), Roux and Rochaix (1911 

 p. 109), Besson (1920 p. 30). 



2242. Mortensen's Peptone Gelatin 



Same as medium 560 but solidified by the 

 addition of gelatin. 



2243. Rossi's Salt Peptone Gelatin 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone 20.0 g. 



3. NaCl 5.0 g. 



4. Gelatin 100.0 g. 



5. Na.COs 0.6 g. 



6. K2CO3 0.9 g. 



7. NaOH or KOH 2.0 to 4.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: To be used in water examination. 

 Reference: Rossi (1893 p. 69). 



2244. Metchnikoff's Nitrate Peptone 



Gelatin (Brau) 



Constituents ; 



1. Water 



2. Gelatin. 



2.0 g. 



