722 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Peptone (dry) 1.0 g. 



4. NaCl 1.0 g. 



5. KNO, 0.1 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 



sufficient quantity of water. 

 Sterilization: Method not given. 

 Use: Isolation of cholera vibrio. The 



author added 200.0 cc. of the water under 



investigation to each flask. 

 Variants: Pergola solidified medium 549 



by the addition of 10.0 to 15.0% gelatin. 

 References: Brau (1905 p. 813), Pergola 



(1911 p. 85). 



2245. Lichtenstein's Cenovis Gelatin 



Same as medium 518 but solidified by the 

 addition of gelatin. 



2246. Utz's Glucose Peptone Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Gelatin 100.0 g. 



3. Peptone 10.0 g. 



4. NaCl 5.0 g. 



5. Glucose 20.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Neutralize. 



(3) Mix with egg white. 



(4) Shake. 



(5) Boil. 



(6) Filter. 



(7) Add 20.0 g. glucose. 

 Sterilization: Not specified. 



Use: Cultivation of organisms found in 

 milk. d'Herelle cultivated the Cocco- 

 bacillus of the grasshopper (Coccobadllus 

 acridiorium). 



Variants: d'Herelle prepared a similar me- 

 dium as follows: 



(1) Dissolve 40.0 g. Chapoteaut's pep- 

 tone, 5.0 g. NaCl, 30.0 g. gelatin and 

 5.0 g. glucose in 1000.0 water by 

 boiling. 



(2) Make slightly alkaline. 



(3) Filter. 



(4) Distribute in bottles. 



(5) Plug with cotton and cover the cotton 

 with a layer of parchment paper. 



(6) Sterilize at 120° for 30 minutes. 



He reported that the virulence of the 

 culture was maintained for about 15 days 

 in this medium. When a culture was 

 powdered due to the gelatin content of 



the medium, it stuck to the plants very 

 well. 

 References: Utz (1903-04 p. 610), d'Herelle 

 (1914 p. 310). 



2247. Beijerinck's Glucose Peptone 

 Gelatin 



Constituents : 



1. Water 100.0 cc. 



2. Glucose 5.0 g. 



3. Peptone 5.0 g. 



4. Gelatin 10.0 g. 



5. Potassium biphosphate 0.02 g. 



Preparation: Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of vinegar bacteria pro- 

 ducing pigment. The author reported 

 that the gelatin was first liquefied and 

 then coagulated. 



Reference: Beijerinck (1911 p. 174). 



2248, Pfeiler and Lentz's Ringer Solution 



Peptone Gelatin 



Same as medium 1551 but solidified by 

 the addition of 15.0% gelatin instead 

 of agar. 



2249. Vierling's Glucose Peptone Gelatin 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. CaClz 0.1 g. 



4. MgS04 0.1 g. 



5. FeClj trace 



6. NaCl trace 



7. Gelatin 100.0 g. 



8. Peptone 10.0 g. 



9. Dextrose 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 



(2) Adjust to a slightly alkaline reaction 

 by the addition of soda. 



Sterilization: Not specified. 



Use: To study proteolytic action, lique- 

 faction by mycobacteria. The author 

 reported that growth was good, but lique- 

 faction did not take place. Same results 

 if glucose be omitted. 



Reference: Vierling (1920 p. 203). 



2250. Sear's Glucose Peptone Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



