CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



723 



3. NaCl 5.0 g. 



4. Gelatin 50.0 g. 



5. Glucose (1.0%) 10.0 g. 



6. CaCO, 

 Preparation : 



(1) D,issolve 2, 3 and 4 in 1. 



(2) Clear with white of egg. 



(3) Neutralize to phenolphthalein. 



(4) Filter till clear. 



(5) Add 1.0% glucose (glucose may be 

 omitted). 



(6) Distribute both portions into 300.0 cc. 

 flasks, 200.0 cc. per flask. 



Sterilization: Sterilize at 15 pounds for 

 10 minutes. 



Use: To study nitrogen metabolism. Am- 

 monia determined by Folin's method, 

 amino acid by Van Slyke's micro method 

 and total nitrogen by Kj,eldahl-Gunning- 

 Arnold method. 



Reference: Sears (1916 p. 126). 



2251. Utz's Lactose Peptone Gelatin 



Same as medium 2246 but used 20.0 g. 

 lactose instead of glucose. 



2252. Molisch's Sucrose Peptone Gelatin 

 (Smith) 



Constituents: 



1. Water 1000.0 cc. 



2. Gelatin 100.0 g 



3. Sucrose 20.0 g 



4. Peptone 10.0 g, 



5. K2HPO4 0.25 g 



6. MgS04 0.25 g, 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Make feebly alkaline by the addition 

 of NaOH. 



Sterilization: Not specified. 



Use: Cultivation of luminous bacteria. 

 Smith reported that on this medium the 

 bacteria grew feebly and were non- 

 luminous until NaCl or some equivalent 

 material was added (3.0%). 



Reference: Smith (1905 p. 198). 



2253. Trommsdorff's Sucrose Peptone 

 Gelatin 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Witte) (0.5%) .... 5.0 g. 



3. Sucrose (0.5%) 5.0 g. 



4. Gelatin (8.0%) 80.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Leptomitus. 

 Reference: Trommsdorff (1918 p. 65). 



2254. Molisch's Dextrin Peptone Gelatin 

 Constituents : 



1. Moldau water 1000.0 cc. 



2. Gelatin lOO.O g. 



3. Peptone 5.0 g. 



4. Dextrin 5.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of purple bacteria. 

 Reference: Molisch (1907 p. 11). 



2255. Matzuschita's Glycerol Peptone 

 Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 5.0 g. 



4. Gelatin 80.0 to 100.0 g. 



5. Glycerol 60.0 g. 



Preparation : 



(1) Prepare ordinary nutrient gelatin 

 using water instead of bouillon. 

 (Peptone and NaCl, in the amounts 

 indicated, were considered to be the 

 constituents of nutrient gelatin. 

 Exact amount of gelatin not specified 

 in the article either.) 



(2) Add 6.0% glycerol to (1). 

 Sterilization: Not specified. 



Use: Cultivation of mammalian and 



chicken tubercle bacilli. 

 Reference: Matzuschita (1899 p. 128). 



2256. Molisch's Glycerol Peptone Gelatin 



Constituents: 



1. Moldau water 1000.0 cc. 



2. Crelatin 100.0 g. 



3. Peptone 5.0 g. 



4. Glycerol 5.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of purple bacteria. 

 Reference: Molisch (1907 p. 11). 



2257. Freudenreich and Jensen's Lactate 



Peptone Gelatin (Boekhout and Ott de 



Vries) 



Same as medium 644 but solidified by the 

 addition of 10.0% gelatin. 



