726 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2. Peat 



3. Peptone, manganese 0.25 g. 



4. Gelatin 100.0 g. 



Preparation : 



(1) Boil a piece of peat brick the size of a 

 fist in a liter of water to prepare the 

 peat infusion. 



(2) Dissolve 2 and 3 in (1). 



(3) Make slightly alkaline by the addi- 

 tion of NaOH. 



Sterilization: Not specified. 



Use: Cultivation of iron bacteria, lepto- 



thrix. 

 Reference: Molisch (1910 p. 39). 



2269. Deycke's Peptone Alkali Albumin 

 Gelatin 



Constituents : 



1. Water 1200.0 cc. 



2. KOH (3.0%) 36.0 g. 



3. Peptone 1.0% 



4. Veal 1000.0 g. 



5. Gelatin 10.0% 



6. NaCl 1.0% 



Preparation : 



(1) Digest 1000.0 g. finely ground and 

 fat-free veal in 1200.0 cc. of 3.0% 

 KOH in flask for 2 days at 37°C. 



(2) Heat one hour on water bath 60- 

 70°C. until all protein dissolves. 



(3) Add HCl carefully to precipitate 

 albuminate. 



(4) Collect on filter. 



(5) Suspend in distilled water and add 

 concentrated soda solution until 

 alkaline reaction brings part into 

 solution. 



(6) Dissolve by prolonged heating in 

 steam. 



(7) Correct reaction to weak alkalinity. 



(8) Determine % dry weight by drying 

 100.0 cc. sample at 100°C. 



(9) Dilute (8) so that there is about 

 3.0% dry material present. 



(10) Add 1.0% peptone, 1.0% NaCl and 

 10.0% gelatin. 



(11) Dissolve and neutralize by Dah- 

 men's method using 0.33% soda 

 solution. 



Sterilization: Sterilize on 3 successive days 

 for 10 minutes each day in streaming 

 steam. 



Use: Isolation of cholera bacilli from 

 stools. The author reported the medium 



eliminated practically all other intestinal 

 organisms. 

 References: Deycke (1893 p. 888), (1895 

 p. 243), Klimmer (1923 p. 220). 



2270. Kotlar's Pancreas Gelatin 



Constituents: 



1. Water 400.0 cc. 



2. Pancreas (calf) 200.0 g. 



3. Peptone (2.0%) 2.0 g. 



4. NaCl (1.0%) 1.0 g. 



5. Gelatin (20.0%) 20.0 g. 



Preparation : 



(1) Add a little water to 200.0 g. of small 

 fat and connective tissue free pieces 

 of calf pancreas and mince to a pulp. 



(2) Add 200.0 cc. of water to (1). 



(3) Filter thru a clay filter. 



(4) Prepare a watery solution of 20.0% 

 gelatin containing 2.0% peptone and 

 1.0% NaCl. 



(5) Mi.x 100.0 cc. of sterile (4) and 100.0 cc. 

 of sterile (3). 



Sterilization: Sterilize (2) by filtering thru 

 a clay filter (see step (3) above). Method 

 of sterilization of (4) not given. 



Use: Show effect of pancreas extract on 

 growth of Bad. coli, Bad. typhosus, 

 cholera bacillus, anthrax bacillus and 

 Staphylococcus pyogenes aureus. Author 

 reported that the organisms were in- 

 hibited by the extract. 



Reference: Kotlar (1895 p. 147). 



2271. Harde's Tissue Gelatin 



Constituents: 



1. Nutrient gelatin. 



2. Beef or veal. 



Preparation: (1) Add a 2 cc. cube of beef or 

 veal to 20.0% gelatin just before steri- 

 lization. 



Sterilization: Method not given. 



Use: To obtain anaerobes and aerobea 

 from exudates. 



Reference: Harde (1917 p. 661). 



2272, Pergola's Nitrate Alkaline Blood 

 Gelatin 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone 10.0 g. 



3. NaCl 10.0 g. 



4. NasCOs 0.2 g. 



5. KNO3 0.1 g. 



