CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



737 



(3) Filter in the autoclave or use a 

 hot water funnel. 



(4) Tube. 



(5) Add magnesium phosphate 

 Cas (PO 4) 2 and CaCO 3 to each tube. 

 (50.0 g. Ca3(P04)2, 5.0 g. mag- 

 nesium phosphate and 10.0 g. 

 CaCO 3 were used for the entire 

 amount of medium.) 



(6) Sterilization not specified. 



(b) Besson prepared a similar medium 

 as follows: 



(1) Remove all fat and tendons from 

 beef and chop into small pieces. 



(2) Allow 500.0 g. of (1) to macerate 

 with 1000.0 cc. of cold water for 

 6 hours, or if one wishes to remove 

 the sugar, 12 hours at 37°C. 



(3) Place in an enamelled pot and 

 bring slowly to a boil. 



(4) Boil for 10 minutes. 



(5) Throw on a thick cloth and press 

 the meat free from juice. 



(6) Filter the juice thru moistened 

 paper. 



(7) Make up to 1000.0 cc. 



(8) Add 10.0 g. Chapoteaut's peptone, 

 5.0 g. NaCl, a pinch of sodium 

 phosphate and 80.0 to 130.0 g. 

 gelatin (80.0 g. in winter, 130.0 g. 

 in summer). 



(9) Heat slowly in an enamelled kettle. 



(10) After solution is complete, boil 

 for 2 or 3 minutes. 



(11) Make slightly alkaline by the 

 addition of soda. 



(12) Heat at 115°C. for 5 minutes. 



(13) Filter thru paper in the autoclave 

 or use a hot water funnel. 



(14) Tube. 



(15) Sterilize at 110° for 20 minutes. 

 References: Roux and Rochai.K (1911 



p. Ill), Besson (1920 pp. 38, 41). 



2284. Miiller's Indicator Infusion Gelatin 



Same as medium 784, but solidified by the 

 addition of gelatin. 



2285. Jackson and Muer's Liver Infusion 



Gelatin 



Constituents: 



1. Water 1000.0 cc. 



2. Liver, beef 500.0 g. 



3. Gelatin 100.0 g. 



4. Peptone (Witte's) 10.0 g. 



5. Glucose 10.0 g. 



6. Potassium phosphate 

 (K2HPO4) 1.0 g. 



Preparation : 



(1) Chop 500.0 g. beef liver into small 

 pieces and add 1000.0 cc. water 

 Weigh the infusion and container. 



(2) Boil blowly for 2 hours in a double 

 boiler, starting cold, and stirring it 

 occasionally. 



(3) Make up the loss in weight by evap- 

 oration and strain thru a wire 

 strainer. 



(4) Cool the filtrate to 50°C. Add 3, and 

 stir a few minutes until dissolved. 



(5) Add 5 and 6. 



(6) Stir until the ingredients are dis- 

 solved, keeping the temperature 

 below 50°C. 



(7) After warming this mixture in a 

 double boiler and stirring it for a few 

 minutes to dissolve ingredients, ti- 

 trate with N/20 sodium hydrate using 

 phenolphthalein as an indicator, and 

 neutralize with normal sodium 

 hydrate. 



(8) Boil vigorously for 30 minutes in a 

 double boiler, and 5 minutes over a 

 free flame with constant stirring to 

 prevent the caramelization of the 

 dextrose. 



(9) Make up any loss in weight by evap- 

 oration and filter thru cotton flannel 

 and filter paper. 



Sterilization: Sterilize in an autoclave for 

 15 minutes at 15 pounds pressure. 



Use: Identification of certain bacteria. 

 Used also for cultivation of B. sporogenes. 



Reference : Jackson and Muer (1911 p. 290), 

 (1911 p. 928). 



2286. Fuhrmann's Glucose Infusion Gelatin 



Constituents : 



1. Horse meat infusion 1000.0 cc. 



2. Gelatin 100.0 g. 



3. Peptone (Witte) 20.0 g. 



4. Dextrose 10.0 g. 



5. NaCl 5.0 g. 



Preparation : 



(1) Prepare horse meat infusion. 



(2) Dissolve 2, 3, 4 and 5 in (1). 



(3) Pour in plates. 

 Sterilization: Not specified. 



