738 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation of Pseudomonas cerevi- 

 siae. The author reported that best 

 growth was obtained when the reaction 

 was 1.0% N/1 alkali. 



Variants: The author gave the following 

 method of preparation: 



(1) Digest 1000.0 g. of horse meat with 

 1 liter of water for 24 hours in the 

 cold. 



(2) Boil for one hour in the steamer. 



(3) Cool and filter. Make up to 1 liter. 



(4) Add 40.0 g. of Witte's (siccum) pep- 

 tone, 20.0 g. glucose, and 10.0 g. of 

 NaCl. (This is a stock bouillon.) 



(5) To 500.0 cc. of (4) and 100.0 g. gelatin. 



(6) Neutralize and clarify. 



(7) Dilute with an equal volume of water. 



(8) Add N/1 acetic acid or N/1 NaOH to 

 obtained the desired reaction. 



(9) Sterilization not specified. 

 Reference: Fuhrmann (1906 pp. 310, 316). 



2287. Choquet's Glycero Phosphate 

 Infusion Gelatin (Besson) 

 Constituents : 



1. Beef juice 300.0 cc. 



2. Gelatin (extra white) 35.0 g. 



3. Peptone (Chapoteaut) 5.0 g. 



4. Glycerol-phosphate of lime. . 5.0 g. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in beef juice. 



(2) Heat in the autoclave. 



(3) Filter hot in the autoclave or use a 

 hot water funnel. 



(4) Tube. 

 Sterilization: Not specified. 



Use: Cultivation of organisms from dental 



caries. 

 Reference: Besson (1920 p. 40). 



2288. Teague and Clurman's Congo-Red 



Brilliant Green Glucose Gelatin 

 Constituents : 



1. Infusion gelatin (5.0%) . . . 500.0 cc. 



2. Congo red (2.0%) 40.0 cc. 



3. Brilliant green 0.415 g. 



4. Glucose (0.5%) 2.5 g. 



5. Bromoform 

 Preparation : 



(1) E.xact method of preparation of 5.0% 

 nutrient gelatin or composition not 

 given. It is to be prepared however 

 from meat infusion, not from beef 

 extract, and the reaction to be 1.0+. 



(2) Prepare a 2.0% solution of congo red. 



(3) Add 4.0 cc. of (2) to every 50.0 cc. of 

 (1), also add 0.83% brilliant green 

 and 0.5% glucose. 



(4) Add 25.0 cc. of bromoform to 100.0 cc. 

 of sterile distilled water. 



(5) Shake thoroly and allow to stand at 

 room temperature over night. 



(6) Pipette some of the saturated solu- 

 tion into a sterile flask (do not dis- 

 turb the bromoform at the bottom of 

 the flask, do not include any of the 

 globules floating on the surface). 



(7) One cc. of (6) (saturated bromoform 

 solution) is added to each 10.0 cc. of 

 (3) , (congo-red-brilliant-green-gela- 

 tin) just before use. 



Sterilization: Not specified. 



Use: Enrichment medium for typhoid 



organisms. 

 Reference: Teague and Clurman (1916-17 



p. 125). 



2289. Kowalski's Glycerol Lung Infusion 



Gelatin 

 Constituents : 



1. Water. 



2. Lung, calf. 



3. NaCl. 



4 Potassium phosphate. 



5. NaSOi. 



6. (NH4)2S04. 



7. Sucrose. 



8. Peptone. 



9. Gelatin (10.0 to 15.0%). 

 10. Glycerol (8.0 to 10.0%). 



Preparation : 



(1) Grind one kilogram fresh calf lung. 



(2) Add 2 liters water. Boil in glass 

 vessel 30 minutes. 



(3) Filter, pressing the meat. 



(4) Dissolve 3, 4, 5, 6, 7 and 8 in (3). 



(5) Add 10-15.0% gelatin. 



(6) Dissolve by heat. 



(7) Neutralize with equal parts of 

 sodium and potassium hydrate. 



(8) Make to 2.5 liters with distilled 

 water. 



(9) Cool to 58°C. 



(10) Add the beaten whites of 4 eggs. 



(11) Heat a few minutes. 



(12) Filter. 



(13) Add 8-10.0% glycerol to filtrate. 



(14) Distribute in tubes or flasks. 



