746 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add neutral red to give a brilliant 

 color to (1). 



Sterilization: Not specified. 



Use: To determine fluorescence production 



by organisms found in ice cream. 

 Reference: Buchan (1910 p. 108). 



2302. von Caron's Nitrate Extract Gelatin 

 Constituents : 



1. Water 1000.0 cc. 



2. Peptone (1.0%) 10.0 g. 



3. Beef extract (Lie- 

 big's) (1.0%) 10.0 g. 



4. KNO3 2.0 g. 



5. Gelatin (5.0 to 



10.0%) 50.0 to 100.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 



Sterilization: Not specified. 



Use: Isolation of denitrifying bacteria. 

 Bac. Stutzeri, Bac. filefaciens, Bad. deni- 

 trificans, Vibrio denitrificans, Bad. 

 nitrovorum, Bad. centropundatum. 



Variants: Percival dissolved 0.5% Lemco or 

 other meat extract, 1.0% peptone, 0.0 or 

 0.5% NaCl, 0.3 g. NaNOa and 10.0% gelatin 

 in water. The medium was neutralized 

 by the addition of Na2C03 and sterilizer 

 on 3 successive days for 20 minutes. 



References: von Caron (1912 p. 70), Perci- 

 val (1920 p. 165). 



2303. Henneberg's Glucose Extract Gelatin 

 Constituents : 



1. Water 1000.0 cc. 



2. Dextrose 20.0 to 50.0 g. 



3. Meat extract 10.0 g. 



4. Peptone 10.0 g. 



5. NaCl 2.0 g. 



6. Gelatin 50.0 to 100.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use: Cultivation of acetic acid bacteria 

 B. Pasteurianum, B. oxydans, B. aceti, 

 B. Kiitzingianum. Banning used a sim- 

 ilar medium to study oxalic acid forma- 

 tion by Bad. industrium Henneberg, 

 Bad. oxydans Henneberg, Thermobac- 

 terium aceti Zeidler, Bad. acidi oxalic, 

 Bad. monasteriense, Bad. diabdicum, 

 Bad. dortmundense, and Bad. parvulum. 

 Agar was used to grow those organisms 



that liquefy gelatin. Oxalic acid was 

 formed by these organisms. Wash a cul- 

 ture from the surface of the agar or gela- 

 tin with H2SO4. Filter and evaporate. 

 Oxalic acid crystals will form. 



Variants: Banning prepared a medium by 

 dissolving 20.0 g. glucose, 10.0 g. peptone, 

 10.0 g. meat extract and 70.0 g. gelatin 

 in 1000.0 cc. water. The reaction was 

 slightly acid. 



References: Henneberg (1898 p. 18), Ban- 

 ning (1902 p. 397, 426). 



2304. Gottheil's Glucose Extract Gelatin 

 Constituents : 

 1- Water lOOO.O cc. 



2. Gelatin loo.O g. 



3. Glucose 10.0 g. 



4. NaCl [.', 2*0 g! 



5. Meat extract (Liebig's) .... 8.0 g. 



6. Peptone (Witte) 12.0 g. 



Preparation : 



(1) Dissolve 4, 5 and 6 in 500.0 cc. of 

 water. 



(2) Neutralize by the addition of a con- 

 centrated Na2C03 solution. 



(3) Heat for a time in a sterilizer. 



(4) Filter. 



(5) Soak 100.0 g. gelatin in 500.0 cc. of 

 water for about 3 hours. 



(6) Add (4) to (5). 



(7) Allow to stand for 3 hours at 100°C. 



(8) Neutralize once more with Na2C03 

 and heat for a short time. 



(9) Filter. 



(10) Add 10.0 g. glucose. 



Sterilization: Sterilize on 3 successive days 

 in the steamer. 



Use: Gottheil cultivated organisms found 

 in the soil and in the root and rhizomes of 

 leguminous plants. Bachman cultivated 

 obligate anaerobes on a similar medium. 



Variants: Bachman dissolved 16.0% gela- 

 tin, 1.0% glucose, 1.0% Witte's peptone, 

 0.8% meat extract, and 0.2% NaCl in 

 water. The reaction was adjusted to a 

 slight alkalinity by the addition of 

 Na.COa. 



References: Gottheil (1901 p. 432), Bach- 

 man (1912-13 p. 7). 



2305. Buchner's Sucrose Extract Gelatin 

 Constituents : 

 1. Water 1000.0 cc. 



