CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



747 



2. Gelatin 100.0 g. 



3. Liebig's meat extract 5.0 g. 



4. Peptone (puriss) 5.0 g. 



5. Sucrose 20.0 g. 



6. NasHPOi 5.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Make slightly alkaline by the addition 

 of soda. 



Sterilization: Not specified. 



Use: Cultivation of cholera bacillus. 



Variants: Besson used 5.0 g. Ca3(P04)2 



instead of Na2HP04. 

 References: Buchner (1885 p. 368), Weis- 



ser (1886 p. 325), Besson (1920 p. 40). 



2306. Percival's Urea Extract Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Beef extract (Lemco) 5.0 g. 



3. Peptone (Witte) 10.0 g. 



4. Gelatin 100.0 g. 



5. Urea 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) If acid neutralize with (NH4)2C03. 



(3) Tube. 



Sterilization: Method not given. 



Use: Cultivation of urea splitting organ- 

 isms, Urobacillus Pasteuri. The author 

 reported that urea fermenting colonies 

 were surrounded with small white crys- 

 tals of calcium carbonate. 



Reference: Percival (1920 p. 224). 



2307. Zipfel's Basal Nutrient Gelatin 



Constituent: 



1. Nutrient gelatin 1000.0 cc. 



Preparation: (1) Prepare nutrient gelatin 

 and dissolve one of the added nutrients. 



Sterilization: Not specified. 



Use : Zipfel cultivated nodule bacteria. 



Added nutrients : The author added one of 

 the following: 



Leucine 2.0 or 3.0% 



Tyrosine 0.05, 0.1 or 0.2% 



Cholesterin. . . . 0.02, 0.3 or 0.5% 



Asparagin 2.0 or 5.0% 



Nucleic acid. . . 0.2, 0.5, 1.0 or 2.0% 

 Caffeine 0.1, 0.2, 0.3, 0.4 or 0.5% 



Variants : Besson added 2.0 to 4.0% of any 

 desired carbohydrate, alcohol, etc., to 

 nutrient gelatin. Just before use the 

 medium was melted and sufficient quan- 



tity of litmus added to give a light blue 

 color. 

 References: Zipfel (1911-12 p. 129), Besson 

 (1920 p. 59). 



2308. Wurtz's Nutrient Gelatin 



Constituents: 



1. Bouillon 1000.0 cc. 



2. Gelatin 70.0 g. 



Preparation : 



(1) Add 70.0 g. gelatin to a liter of 

 bouillon. 



(2) Heat on a salt water bath until the 

 gelatin is dissolved. 



(3) Adjust the reaction to a slight 

 alkalinity. 



(4) Add the whites of 2 eggs beaten up in 

 some water, when the temperature 

 of the gelatin reaches 50 °C. 



(5) Heat in the autoclave at 115° for one 

 minute. 



(6) Filter thru Chardin paper. 



(7) Distribute in tubes. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Variants : 



(a) Smith dissolved 10.0 to 15.0% of the 

 best grade of sheet gelatin in bouillon. 



(b) Sergent cultivated Bacterium zopfii 

 on one of the following combinations: 



Bouillon.. 1000.0 cc. 1000.0 cc. 1000.0 cc. 

 NaCl .... 5.0 g. 5.0 g. 

 Peptone.. 10.0 g. 10.0 g. 10.0 g. 

 Gelatin... 130.0 g. 80.0 g. 180.0 g. 



(c) Lohnis prepared the medium as 

 follows: 



(1) Add 10.0% of the best Gold Label 

 gelatin to 350 cc. hot bouillon. 



(2) When solution is complete neu- 

 tralize to litmus. 



(3) Steam for 45 minutes. 



(4) Readjust the reaction if necessary. 



(5) Beat the white of an egg thoroly. 



(6) Add (5) to (4) cooled to about 



50°C. 



(7) Heat for 30 to 45 minutes in the 

 steamer. 



(8) Filter thru folded filter paper 

 using a hot water funnel. 



(9) Distribute as desired. 



(10) Steam for 10 to 15 minutes. 



(d) Roddy gave the following method of 

 preparation: 



(1) Break up 100 to 300.0 g. of the best 



