750 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization: Method not given. 

 Use: Cultivation of tubercle bacilli. 

 Reference: Wurtz (1897 p. 46). 



2314. Holz's Phenol Gelatin 

 Constituents: 



1. Nutrient gelatin. 



2. Phenol (5.0% solution). 

 Preparation : 



(1) Prepare nutrient gelatin in the usual 

 manner. 



(2) Neutralize with 10.0% NaoCOs solu- 

 tion, using litmus as an indicator. 



(3) Distribute sterile (2) in 10.0 cc. lots 

 by means of a burette. 



(4) Heat once more. 



(5) Add from 7 to 12 drops of 5.0% phenol 

 solution to each tube. (3 drops = 

 0.1 cc.) 



(6) Pour in plates, or prepare roll 

 cultures. 



Sterilization: Sterilize (2) by repeated 



heatings. 

 Use: Isolation of typhoid bacilli. 

 Variants: Migula added 0.5 cc. of a 4.0% 



phenol solution to each tube (10.0 cc.) 



of slightly alkaline nutrient gelatin. 

 References: Holz (1890 p. 144), Migula 



(1901 p. 24). 



2315. Stutzer and Hartleb's Urea Gelatin 

 Constituents: 



1. Nutrient gelatin 1000.0 cc 



2. Urea (2.0%) 20.0 g. 



Preparation : 



(1) Add 2.0% urea to ordinary nutrient 

 gelatin. 



Sterilization: Method not given. 



Use: Attempt to cultivate the bacterium 

 of foot and mouth disease. Other in- 

 vestigators cultivated soil bacteria and 

 urea splitters on similar media. 



Variants : 



(a) Hoffman added 1.0% urea to 10.0% 

 alkaline gelatin. The medium was 

 used for differential count of bacteria 

 from soil. He reported that if urea 

 was fermented the colonies were 

 surrounded by a halo of characteris- 

 tic biscuit shaped crystals. 



(b) Lohnis studied urea decomposition 

 on a medium prepared by adding 

 1.0 cc. of a 15.0% aqueous solution 

 of urea to tubes of gelatin. This 



medium was then heated in the steam 

 sterilizer. 



(c) Geilinger cultivated urea splitting 

 organisms on nutrient gelatin con- 

 taining 1.0% urea. 



(d) Cunningham determined the de- 

 composition of urea on nutrient 

 12.0% gelatin containing 2.0% urea. 

 The medium was sterilized by the 

 intermittent method in the steamer. 



References: Stutzer and Hartleb (1897 p. 

 403), Hoffman (1912 p. 387), Lohnis 

 (1913 p. 95), Geilinger (1917 p. 246), 

 Cunningham (1924 p. 143). 



2316. Park, Williams and Krumwiede's 

 Meat Gelatin 

 Constituents : 



1. Nutrient gelatin. 



2. Meat. 

 Preparation : 



(1) Drop pieces of meat into tubes of 

 nutrient gelatin. 

 Sterilization: Method not given. 

 Use: General culture medium. 

 Reference : Park, Williams and Krumwiede 



(1924 p. 125). 



2317. Worth's Glucose Liver Gelatin 

 Constituents : 



1. Nutrient gelatin 1000.0 cc. 



2. Glucose (2.0%) 20.0 g. 



3. Liver, rabbit 

 Preparation : 



(1) Prepare nutrient gelatin and add 2.0% 

 glucose. 



(2) Adjust the reaction to -f-1.0% acid. 



(3) Add rabbit liver. 

 Sterilization: Not specified. 

 Use: Preservation of cultures. 

 Reference: Worth (1919 p. 604). 



2318. Nastinkoff's Egg Yolk Nutrient 

 Gelatin 



Same as medium 1950 but solidified with 

 gelatin instead of agar. 



2319. Pergola's Alkaline Blood Gelatin 

 Constituents : 



1. Nutrient gelatin 700.0 cc. 



2. Blood, beef 150.0 cc. 



3. KOH, normal 150.0 cc. 



Preparation : 



(1) Collect beef blood in a sterile flask 



