CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



755 



Preparation : 



(1) Soak 250.0 g. of dried raisins in a 

 liter of water for several hours. 



(2) Boil. 



(3) Pass thru a coarse sieve. 



(4) Filter thru paper. 



(5) Dissolve 100.0 g. gelatin and a pinch 

 of sodium phosphate in the filtrate. 



(6) Boil for 2 or 3 minutes. 



(7) Make slightly alkaline by the 

 addition of soda. 



(8) Heat at 115° for 5 minutes. 



(9) Filter thru paper, in the autoclave or 

 use a hot water funnel. 



(10) Tube. 

 Sterilization: Sterilize at 110° for 20 



minutes. 

 Use: General culture medium. 

 Reference: Besson (1920 p. 40). 



2334, Klimmer's Prune Infusion Gelatin 



Constituents: 



1. Water 500.0 cc. 



2. Prunes 100.0 g. 



3. Gelatin 

 Preparation : 



(1) Boil 100.0 g. of dried prunes in 500.0 

 cc. of water, 



(2) Filter. 



(3) Add gelatin (amount not given). 



(4) Boil. 



(5) Filter. 



(6) Distribute as desired. 

 Sterilization: Method not given. 

 Use: General culture medium. 

 Reference: Klimmer (1923 p. 206). 



2335, Lohnis' Plum Extract Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Plums 1000.0 g. 



3. Gelatin (10.0%) 100.0 g. 



Preparation : 



(1) Boil 1000.0 g. of plums with 1000.0 cc. 

 of water twice. 



(2) Add 10.0% gelatin to the juice of (1) 

 without neutralization. 



Sterilization: Not specified. 

 Use: Cultivation of molds. 

 Reference: Lohnis (1918 p. 88). 



2336. Eisner's Iodide Potato Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Potato 500.0 g, 



3. Gelatin 



4. KI (1.0%) 10.0 g. 



Preparation : 



(1) Prepare a gelatin using 500.0 g. 

 potatoes and 1 liter of water. 

 Amount of gelatin to be added or 

 method of preparation not given. 



(2) Add N/1 NaOH to neutralize to 

 litmus. 



(3) Filter and flask. 



(4) Add 1.0% KI to each flask of sterile 

 (3). 



Sterilization: Method of sterilization of 

 (3) not given. 



Use: Differentiation of coli and typhoid 

 bacteria. The author reported that B. 

 coli grew rapidly and luxuriantly. Colo- 

 nies were brown. Typhoid organisms 

 grew slower. After 48 hours colonies 

 were small and glistening resembling 

 drops of water. 



Variants : 



(a) Grimbert gave the following method 

 of preparation: 



(1) Grate 500.0 g. potatoes and allow 

 to macerate with one liter of water 

 for 3 or 4 hours or better allow to 

 stand over night in the cold. 



(2) Decant and filter. 



(3) Autoclave the filtrate for 10 

 minutes, 



(4) Filter, 



(5) Add 15.0 g, of gelatin for each 

 100.0 cc. of the filtrate, and melt 

 by heating on the salt water bath. 



(6) Cool to 55°C. and add the white of 

 an egg suspended in a little water. 



(7) Adjust the reaction so that 5.0 cc. 

 of lime solution is required to 

 neutralize 10.0 cc. medium to 

 phenolphthalein. (An acidity 

 equivalent to 1.0 g. H2SO4 per 

 liter.) 



(8) Heat in the autoclave for 10 

 minutes at 110°C. to clarify. 



(9) Filter. 



(10) Tube in 9.0 cc. lots. 



(11) Sterilize in the autoclave at 110° 

 for 15 minutes. 



(12) When ready for use add 1.0 cc. of 

 a sterile 10.0% KI solution to each 

 tube. 



