758 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Prepare glycerol potato broth. 



(2) Add 100.0 g. of gelatin to (1). 



(3) Boil. 



(4) Adjust the reaction. 



(5) Filter. 



Sterilization: Method not given. 

 Use: General culture medium. 

 Reference: Tanner (1919 p. 60). 



2339. Zikes' Carrot Infusion Gelatin 



Same as variant (c) medium 2337 but 

 using carrots instead of potatotes. 



2340. Stutzer, Burri and Maul's Glucose 

 Mustard Seed Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. White mustard seed 400.0 g. 



3. Glucose 20.0 g. 



4. Gelatin 100.0 g. 



Preparation : 



(1) Germinate white mustard seed at 

 30°C. for 3 days. The sprouts are 

 from 1 to 1.5 cm. long. 



(2) Add 1 liter of water to 400.0 g. of (1). 



(3) Steam at 100°C. for 2 hours. 



(4) Filter while hot. 



(5) Mix 500.0 cc. of filtrate with an 

 equal volume of water. 



(6) Dissolve 100.0 g. gelatin and 20.0 g. 



of glucose in (5). 



(7) Finish the preparation in the usual 

 manner. (Method not specified.) 



(8) Adjust the reaction to a slight acidity. 



(9) Distribute in 8.0 cc. lots. 

 Sterilization: Not specified. 



Use: Cultivation of Bacillus radicicola 



and other nodule bacteria. 

 Reference: Stutzer, Burri and Maul (1896 



p. 667). 



2341. Groenewege's Tomato Infusion 

 Gelatin 



Constituents: 



1. Water 100.0 cc. 



2. Tomato 20.0 g. 



3. Gelatin (10.0%) 10.0 g. 



Preparation : 



(1) Boil 20.0 g. of the fresh material of 

 ripe tomatoes in 100.0 cc. of water. 



(2) Filter. 



(3) Neutralize the filtrate to litmus by 

 the addition of NaoCOs. 



(4) Add 10.0% gelatin. 



(5) Clarify and filter. 

 Sterilization: Not specified. 



Use: Cultivation of Phytibacter lyco- 



persicum (causing tomato rot). 

 Reference: Groenewege (1917 p. 24). 



2342. Will's Cabbage Juice Gelatin 



Constituents : 



1. Cabbage juice. 



2. Gelatin. 

 Preparation : 



(1) Press the juice from cabbage leaves. 



(2) Solidify (1) by the addition of 10.0% 

 gelatin. 



Sterilization: Not specified. 

 Use: Cultivation of mycoderma. 

 Reference: Will (1910 p. 3). 



2343. MuUer-Thurgau's Grape Vine 



Infusion Gelatin 



Same as medium 1188 but solidified by the 

 addition of gelatin. 



2344. de Rossi's Glucose Leguminous Plant 



Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Leguminous plant 



3. Gelatin 



4. Glucose (2.0%) 

 Preparation : 



(1) Prepare a gelatin from the extract of 

 leguminous plant leaves. (Method 

 not given.) 



(2) Add 2.0% glucose. 



(3) Distribute in tubes. 

 Sterilization: Method not given. 

 Use: To study nitrogen fixation. 

 Reference: de Rossi (1909, 1910 p. 271). 



2345. Simon's Asparagin Legume Extract 

 Gelatin (Klimmer and Kriiger) 



Constituents : 



1. Water 1000.0 cc. 



2. Gelatin 110.0 g. 



3. Legume extract 2.0 cc. 



4. Asparagin 2.0 g. 



5. Glucose 10.0 g. 



6. Malic acid 

 Preparation : 



(1) Prepare the legume extract by pour- 

 ing hot water over finely chopped 

 straw of the legumes, mix well, 



