CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



759 



macerate for 2 days and then boil an 

 hour. Pour off the liquid, filter and 

 evaporate until it contains 10.0% 

 dry material. 



(2) Dissolve 2, 3, 4 and 5 in 1. 



(3) Add 2.0 cc. of (1) to (2). 



(4) Neutralize by the addition of NaOH 

 using litmus as an indicator. 



(5) Add malic acid to give a distinct acid 

 reaction. 



Sterilization: Not specified. 



Use: Cultivation of nodule bacteria from 



leguminous plants. 

 Variants: Klimmer prepared the medium 



using citric acid to adjust the reaction 



instead of malic acid. 

 References: Klimmer and Kriiger (1914 p. 



258), Klimmer (1923 p. 228). 



2346. Zipfel's Glucose Legume Infusion 



Gelatin 



Same as medium 2140 but solidified with 

 150.0 g. gelatin instead of 30.0 g. of agar. 



2347. Kaufman's Jequirity Seed Gelatin 



Same as medium 2132 but solidified with 

 15.0% gelatin instead of 1.5 to 2.0% agar. 



2348. Stutzer, Burri and Maul's Glucose 



Alfalfa Gelatin 

 Constituents : 



1. Water 1000.0 cc. 



2. Alfalfa 400.0 g. 



3. Glucose 20.0 g. 



4. Gelatin 100.0 g. 



5. Na2C03 1.0 g. 



Preparation : 



(1) Pour 1 liter of water over 400.0 g. 

 green alfalfa without roots. 



(2) Add 20.0 g. glucose. 



(3) Steam for 90 minutes. 



(4) Filter. 



(5) Add 100.0 g. gelatin to the filtrate 

 and proceed to prepare a sterile medi- 

 um in the usual manner. (Method 

 not specified.) 



(6) Do not neutralize but add 1.0 g. 

 Na2C03 (Reaction slightly acid). 



(7) Tube in 8.0 cc. lots. 

 Sterilization : Not specified 



Use: Cultivation of Bacillus radicicola 



and other nodule bacteria. 

 Reference: Stutzer, Burri and Maul (1896 



p. 666). 



2349. Barthel's Basal Lupini Extract 

 Gelatin 

 Constituents : 



1- Water goQ.O cc, 



2. Gelatin lOO.O g. 



3. Sucrose 5.0 g. 



4. Lupine extract 2.5 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the added nutrients. 



(3) Tube in 10.0 cc. lots. 

 Sterilization: Sterilize the fractional 



method. 

 Use: To study formation of bacteroids by 



Bacillus radicicola and other legmnes. 

 Added nutrients: The author used the 



following materials: 



(a) 0.1, 0.3 or 0.5%, caffeine ground up 

 with water and gum arable. 



(b) 0.1, 0.3 or 0.5 g. guanine ground up 

 with a little water and gum arable. 



(c) 0.1, 0.3 or 0.5 g. guanidine from a 

 watery solution. Acidify slightly by 

 the addition of tartaric acid. 



(d) 0.1, 0.3 or 0.5% pyridine from a 

 watery solution. 



(e) 0.1, 0.3 or 0.5% chinoline from an 

 alcoholic solution. 



Reference; Barthel (1921 p. 638). 



2350. Nastiukoff's Egg Yolk Gelatin 



Same as medium 2144 but solidified with 

 80.0 to 100.0 g. gelatin instead of 15.0 to 

 20.0 g. agar. 



2351. Deycke and Voigtlander's Albuminate 

 Gelatin 



Same as medium 2034 but solidified with 

 gelatin instead of agar. 



2352. Raskin's Whey Albumin Gelatin 

 Constituents : 



1. Whey 1000.0 cc. 



2. Albumin 100.0 g. 



3. Gelatin 60.0 to 100.0 g. 



Preparation : 



(1) Stir thoroly with a glass rod, the 

 albumin of fresh eggs in a flat bot- 

 tomed dish, and add, drop by drop, 

 concentrated NaOH until a solid 

 opaque gelatinous material is 

 obtained. 



