CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



761 



2357. Heinemann's Whey Gelatin 



Constituents : 



1. Whey 1000.0 cc. 



2. Gelatin (10.0%) 100.0 g. 



Preparation: (1) Solidify clarified whey by 



the addition of 10.0% gelatin. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Variants : 



(a) Bobilioff-Preisser cultivated Oospora 

 on a similar medium. They reported 

 that the gelatin was liquefied. 



(b) Meier made bacterial counts of milk 

 and whey on a medium prepared as 

 follows: 



(1) Dissolve 110.0 g. gelatin and 5.0 g. 

 NaCl in 500.0 cc. water. 



(2) Mix equal parts of whey and (1). 



(3) Neutralize by the addition of 

 KOH. Add KOH until turmeric 

 paper is turned quite weakly 

 brownish red. 



(4) Sterilization not specified 



(c) Klimmer prepared the medium as 

 follows: 



(1) Coagulate the casein from milk by 

 heating at 40 °C. in the presence of 

 rennet. 



(2) Separate the whey from the casein. 



(3) Solidify by the addition of gelatin. 



(4) Sterilization not specified. 

 References : Heinemann (1905 p. 128), Bob- 

 ilioff-Preisser (1916 p. 400), Meier (1918 

 p. 435), Tanner (1919 p. 55), Klimmer 

 (1923 p. 172). 



2358. Raskin's Casein Whey Gelatin 

 (Peifer) 



Constituents : 



1. Water 150.0 cc. 



2. Casein (8.0%) 12.0 g. 



3. Whey 350.0 cc. 



4. Gelatin 42.0 g. 



Preparation : 



(1) Prepare an 8.0% solution of pure 



fat-free casein. 



(2) Prepare a filtered mixture of whey 

 with 12.0% gelatin. 



(3) Mix 150.0 cc. of (1) with 350.0 cc. of 

 (2). 



(4) Heat to 60°C. for 15 minutes. 



(5) Distribute into sterile containers. 

 Sterilization : Not specified. 



Use: Cultivation of pathogenic organisms. 

 References: Raskin (1887 ji^43), Abst. by 

 Peifer (1888 p. 568). 



2359. Piorkowski's Urine Infusion Gelatin 



(Scholz and Krause) 



Constituents : 



1. Urine. 



2. Meat water gelatin. 

 Preparation: 



(1) Collect urine under aseptic conditions 

 from a healthy individual. Specific 

 weight of the urine to be 1.015 to 

 1.020. It is slightly alkaline or 

 neutral in reaction. 



(2) Inoculate with a loop of Micrococcus 

 urea (liquefac. or non liquefac.) 

 from a glycerol agar culture. 



(3) Incubate for 24 to 48 hours at 22°C. 



(4) After this time the reaction is slightly 

 alkaline. 



(5) Filter. 



(6) Distribute in tubes in 5 to 10.0 cc. 

 lots. 



(7) Add to each tube of sterile (6) 5.0 to 

 10.0 cc. of a sterile 10.0% meat water 

 gelatin (exact method of preparation 

 not given) under aseptic conditions. 



Sterilization: Sterilize (6) in the steamer at 

 100°C. on 3 successive days for 10 minutes. 

 Method of sterilization of gelatin not 

 given. 



Use: Diagnosis of typhoid fever. 



Reference: Scholz and Krause (1900 p. 432). 



2360. Kutscher's Meat Infusion Gelatin 



Constituents : 



1. Meat Infusion 1000.0 cc. 



2. Gelatin (10.0%) 100.0 g. 



Preparation : 



(1) Prepare meat infusion. 



(2) Dissolve 10.0% gelatin in 1 by boiling 

 over a free flame. 



(3) Add soda solution until the reaction 

 is only slightly alkaline. 



(4) Filter. 



Sterilization: Method of sterilization not 



given. 

 Use: Isolation and cultivation of Spirillum 

 undula majris. Also used by other 

 investigators in making bacterial counts. 

 Variants : 



(a) Fuller and Johnson made bacterial 

 counts in water analysis on a medium 



