762 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



prepared by dissolving 120.0 g. of 

 gelatin in 1000.0 cc. of meat infusion 

 (contains no peptone or salts), 

 (b) Meier used the following preparation 

 for the bacterial count of milk: 



(1) Boil 500.0 g. of fat and tendon free 

 beef in 1 liter water. 



(2) Filter. 



(3) Dissolve 10.0 g. gelatin and 5.0 g. 

 NaCl in the filtrate. 



(4) Neutralize by the addition of 

 KOH. Add KOH until turmeric 

 paper is turned quite weakly 

 brownish red. 



(5) Sterilization not specified. 

 References : Kutscher (1895 p. 615), Fuller 



and Johnson (1899-1900 p. 85), Meier 

 (1918,-p. 435). 



2361. Graham-Smith's Heart Infusion 

 Gelatin 



Same as medium 1342 but solidified with 

 gelatin. 



2362.'^Beijerinck's Asparagin Meat Infusion 

 Gelatin (Ludwig) 



Constituents : 



1. Sea water meat infusion — 1000.0 cc. 



2. Glycerol 10.0 g. 



3. Asparagin 5.0 g. 



4. Gelatin 80.0 g. 



Preparation : 



(1) Exact method of preparation of meat 

 infusion not given. To be prepared 

 from sea water. 



(2) Dissolve 2, 3 and 4 in (1). 

 Sterilization : Method not given. 



Use: Cultivation of photogenic bacteria. 



{Ph. Pfugeri, Ph. phosphorescence, Ph. 



indicum and Ph. luminosum). 

 References: Ludwig (1890 p. 617), Beijer- 



inck (1890 p. 617). 



2363. Kopp's Thyroid Infusion Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Thyroid glands, sheep 500.0 g. 



3. Gelatin 100.0 g. 



4. NaCl 5.0 g. 



Preparation : 



(1) Free sheep thyroid glands from fat 

 and chop the glands into small pieces. 



(2) Extract the glands for 3 hours with 

 an equal weight of sterile water. 



(3) Add (2) to moistened linen and press 

 the fluid thru. 



(4) Prepare a 20% watery gelatin solution 

 containing 1.0% NaCl. 



(5) Mix equal volumes of (4) and sterile 

 (3). 



Sterilization: Filter (3) thru a clay filter to 

 sterilize. Sterilization of gelatin not 

 given. 



Use: Cultivation of colon typhoid bacteria. 



Reference: Kopp (1895 p. 81). 



2364. Wroblewski's Suprarenal Capsule 

 Infusion Gelatin 



Constituents : 



1. Water 500.0 cc. 



2. Suprarenal capsule (ox) 100.0 g. 



3. Gelatin ' 60.0 g. 



4. NaCl 3.0 g. 



Preparation : 



(1) Remove the suprarenal capsule from 

 an ox immediately after death. 



(2) Chop into small bits and rub to a 

 pulp. 



(3) To 100.0 g. of (2) add 200.0 cc. of 

 water. 



(4) Make (3) alkaline with soda and boil 

 for 2 hours. (Filtration not spe- 

 cified.) 



(5) Dissolve 60.0 g. of gelatin and 3.0 g. 

 NaCl in 300.0 cc. of water. 



(6) Mix equal quantities of (5) and (4). 



(7) Sterilize. (Method not given.) 



(8) Filter 2 or 3 times to clarify. 



(9) Sterilize again (method not specified). 

 Sterilization: Sterilize (9) method not 



given. Store at 36°C. for several days to 



test sterility. 

 Use: Cultivation of Bad. coli, Bad. 



cholerae asiat., anthrax, prodigiosus and 



pyocyaneus. 

 Reference: Wroblewski (1896 p. 529). 



2365. Harde and Hauser's Fish Infusion 

 Gelatin 



Same as medium 1235 but solidified with 

 15.0% gelatin. 



2366. Molisch's Meat Extract Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Meat extract 0.5 g. 



3. Gelatin (4.5%) 45.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 



