CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



763 



Sterilization: Not specified. 



Use: Cultivation of iron bacteria, Clado- 



ihrix dichotoma Cohn. 

 Variants : Zikes used 0.5% meat extract. 

 References: Molisch (1910 p. 31), Linde 



(1913 p. 373), Zikes (1915 p. 543), Besson 



(1920 p. 40). 



2367. HoUborn's Meat Extract Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Meat extract 16.0 g. 



3. Gelatin 100.0 g. 



4. K3PO4 1.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Make weakly alkaline by the addition 

 of NaOH. 



Sterilization: Method of sterilization not 

 given. 



Use: Cultivation of organism causing Alo- 

 pecia areata (baldness) Trichophyton 

 todens. 



Reference: Hollbom (1895 p. 110). 



2368. Fremlin's Soil Infusion Gelatin 



Constituents : 



1. Soil infusion 1000.0 cc. 



2. Gelatin (10.0%) 100.0 g. 



Preparation : 



(1) Prepare soil infusion. 



(2) Dissolve 10.0% gelatin in (1). 

 Sterilization: Method not given. 

 Use: Cultivation of nitroso bacteria. 

 Variants: Lohnis prepared the medium as 



follows: 



(1) Heat 1000.0 g. of good rich garden 

 soil with a liter of tap water for 30 

 minutes in the autoclave under pres- 

 sure of 1 atmosphere or boil with 2 

 liters of water over a free flame. 



(2) Pour off the turbid liquid. 



(3) Mix talc with the liquid. 



(4) Filter thru a double filter paper. 



(5) Make up the volume to 800.0 cc. if 

 necessary. 



(6) Solidify by the addition of gelatin, 



(7) Sterilize. (Method not given.) 

 References: Fremlin (1903 p. 373), Lohnis 



(1913 p. 101). 



2369. Conn's Glucose Soil Infusion Gelatin 



Constituents : 

 1. Water 1000.0 cc. 



2. Gelatin (12.0%) 120.0 g. 



3. Soil infusion (20.0%) 200.0 cc. 



4. Glucose (0.1%) 1.0 g. 



Preparation : 



(1) Prepare soil infusion by heating soil 

 for one hour at one atmosphere pres- 

 sure, mix with twice its weight of 

 water, allow to stand over night cold, 

 boil 30 minutes and filter. Dilute to 

 contain 0.12 to 0.14% total solids, if 

 necessary. 



(2) Dissolve 12.0% gelatin, 20.0% soil 

 extract and 0.1% glucose in water. 



(3) Add NaOH to adjust the reaction to 

 +0.5 to phenolphthalein. 



Sterilization : Not specified. 

 Use: Bacterial count of soils. 

 Variants: Conn prepared the medium as 

 follows: 



(1) Heat soil in an autoclave at 20 to 25 

 pounds pressure. 



(2) Mix (1) with an equal volume of 

 distilled water and allow to stand in 

 the cold for 12 hours. 



(3) Boil 30 minutes. 



(4) Make up the water lost by evapora- 

 tion. 



(5) Filter. 



(6) Dilute with distilled water to tV 

 its natural strength (900.0 cc. distil- 

 led water 100.0 cc. extract). 



(7) Dissolve 120.0 g. Gold Label Gelatin 

 in 1000.0 cc. of (6). 



(8) Clarify by the use of egg white. 



(9) Adjust to 0.5% normal acid using 

 phenolphthalein as indicator. 



(10) Add 1.0 g. of glucose. 



(11) Tube. 



(12) Sterilization not given. 

 References: Conn (1910 p. 425), (1911-12 



p. 71), (1914 p. 512), (1916 p. 722), Tanner 

 (1919 p. 55). 



2370. Lohnis Cyanamide Soil Infusion 

 Gelatin 



Constituents : 



1. Water 1000.0 cc. 



2. Soil 1000.0 g. 



3. Calcium cyanamide (0.2%). 1.6 g. 



4. Asparagin (0.01%) 0.08 g. 



5. Glucose (0.01%) 0.08 g. 



6. K2HPO4 (0.05%) 0.4 g. 



7. Gelatin (10.0%) 80.0 g. 



