GROUP IV. REVERSIBLY SOLID MEDIA OTHER THAN AGAR 



OR GELATIN 



Ai. Additional constituents, if any, of 

 known chemical composition. 



Marpman's Chondrin Medium 2372 



Lehmann's Caragheen Medium 2373 



Giltner's Butter Medium 2374 



A2. Containing additional constituents of 

 unknown chemical composition. 



Bi. Only one additional constituent of 

 unknown chemical composition. 



Mortensen's Gum Arabic Media 2375 



Schluter's Basal Isinglass Medium . 2376 

 Marpmann Peptone Plant Tissue 



Medium 2377 



Matzuschita's Peptone Konbu 



Medium 2378 



Puccinelli's Infusion Fucus Medium 2379 

 Jacobi's Peptone Fucus Medium... . 2380 

 Jacobi's Infusion Broth Fucus 



Medium 2381 



Celli's Bouillon Fucus Medium 2382 



2372. Marpman's Chondrin Medium 



Constituents : 



1. Water. 



2. Cartilage or ear muscles. 

 Preparation : 



(1) Boil ripe pieces of cartilage or ear 

 muscle in water under an atmosphere 

 pressure in an autoclave, or in small 

 amounts in a pressure flask which 

 stand in 100.0% solution of Na2S04. 



(2) Filter thru a filter paper. 

 Sterilization: Not specified. 

 Use: Substitute for gelatin. 

 Reference: Marpman (1891 p. 123). 



2373. Lehmann's Caragheen Medium 



Constituents : 



1. Distilled water 100.0 cc. 



2. "Caragheen" 5.0 g. 



Preparation : 



(1) Boil 5.0 g. of "caragheen" in 100.0 

 cc. of distilled water for about 30 to 

 60 minutes. Stir. 



(2) Cool and make up the loss of water by 

 adding distilled water. 



(3) Heat to boiling again and filter thru 

 cotton or a usual filter in the steamer. 



(4) In order to hasten filtration, a 1 or 

 2.0% extract may be prepared and 

 then concentrated to the desired 

 strength after filtration. 



Sterilization: Not specified. 



Use: Agar substitute for solidification of 

 liquid media. "Caragheen" is a 

 derivative of Chrondrus crispus. 

 5.0% "caragheen" without any addi- 

 tion of nutrients supported the 

 growth of parasitic and saprophytic 

 fungi and air forms. 



Reference: Lehmann (1919 p. 426). 



2374. Giltner's Butter Medium 



Constituents: 



1. Butter. 

 Preparation : 



(1) Melt butter fat at about 100°C. 



(2) Allow the casein to settle. 



(3) Decant the clear fat. 



(4) Place in about 8.0 cc. sterile test 

 tubes. 



Sterilization: Sterilize by the intermittent 



method. 

 Use: To study fat decomposition. Author 



reported that other kinds of fat might be 



similarly treated. 

 Reference: Giltner (1921 p. 365). 



2375. Mortensen's Gum Arabic Media 



Mortensen's solidified media 207, 329 and 

 560 with gum arable (method not given.) 



2376. Schluter's Basic Isinglass Medium 



Constituents : 



1. Water 250.0 cc. 



2. Isinglass 



3. Peptone (siccum) 1-25 g. 



4. NaCl 1.25 g. 



Preparation: 



(1) Prepare a decoction of isinglass in 

 water by boiling. (Exact method not 

 given). Filter. 



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