766 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(2) Dissolve 3 and 4 in (1). 



(3) Dissolve one of the added nutrients 

 in (2) in any desired concentration. 



(4) Distribute into test tubes, plug and 

 slant. 



Sterilization: Method not specified. 

 Use: To study growth of bacteria on acid 



media. 

 Added nutrients: 



Lactic acid Acetic acid 



Tartaric acid HCl 



Citric acid Alum 



Reference: Schluter (1892 p. 592). 



2377. Marpmann's Peptone Plant Tissue 

 Medium 



Constituents : 



1- Water 700.0 cc. 



2. Glycerol 40.0 g. 



3. Sphaerococcus confervoides . . 30.0 g. 



4. Peptone (liquid Koch) 20.0 g. 



Preparation : 



(1) Macerate 30 parts Sphaerococcus 

 confervoides with 2 parts HCl and 1 

 liter water for two hours. 



(2) Wash with water until the washings 

 no longer turn blue litmus paper red. 



(3) Add to the residue of (2) 700 parts 

 water, 40 parts glycerol, 20 parts 

 peptone (liquid Koch) and 2 parts 

 beaten egg-white. 



(4) Boil for 20 minutes in a steam 

 cylinder. 



(5) Neutralize. 



(6) Filter thru a syrup filter, prepared 

 from a balloon flask with a filter 

 layer of buck-skin and felt. 



Sterilization: Not specified. 



Use: General culture medium. Agar 



substitute. 

 Reference: Marpmann (1891 p. 123). 



2378. Matzuschita's Peptone Konbu 

 Medium 



Constituents : 



1. Water 1000.0 cc. 



2. Konbu 100.0 g. 



3. Peptone, Koch 10.0 g. 



4. Glucose 20.0 g. 



Preparation : 



(1) Cut 100.0 g. of dry Konbu in small 

 pieces and mix with one liter of water. 



(2) Allow to stay in the water for 24 

 hours in the cold. 



(3) Boil for one hour in the steamer 



(4) Filter. 



(5) Add 3 and 4 to the filtrate. 



(6) Boil. 



(7) Neutralize (indicator not specified). 

 Sterilization: Sterilize in the steamer on 



from 2 to 5 successive days for 15 to 30 

 minutes. Incubate for 2 days at 37°C. to 

 test sterility. 



Use: Cultivation of spore forming bacilli, 

 Clostridium hutyricum, Bacillus oedma- 

 tis maligni, Bacillus anthracis sympto- 

 matici, Bacillus sporogenes, Bacillus 

 hotulinus. The Konbu is a Laminaria 

 like Japanese sea weed. 



Reference: Matzuschita (1902 p. 2S7). 



2379. Puccinelli's Infusion Fucus Medium 

 Constituents : 



1. Meat infusion 200.0 g. 



2. Fucus crispus 6.0 g. 



Preparation : 



(1) Method of preparation of meat 

 infusion not given. Neutralize. 



(2) Wash well, 6.0 g. of Fucus crispus in 

 water. 



(3) Boil (2) for one hour in (1) in a water 

 bath or steamer. 



(4) Filter thru a funnel heated by a 

 flame or water funnel. 



(5) Distribute in tubes and sterilize. 

 Sterilization : Method not given. 

 Use: General culture medium. 

 Reference: Puccinelli (1890 p. 405). 



2380. Jacobi's Peptone Fucus Medium 



Constituents : 

 1- Water 1500.0 cc. 



2. Peptone (Kemmerich's) .... 7.5 g. 



3. Peptone (siccum) 15.0 g. 



4. Fucus 37.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 by heating 

 over a free flame. 



(2) Make up to the original volume by 

 addition of water. 



(3) Press thru a towel, for some of the 

 fucus will not dissolve. 



(4) Add Na2C03 or sodium phosphate 

 until the reaction is slightly alkaline. 



(5) Pour into a flask and steam until the 

 albuminous material still to be 

 removed, is completely separated. 



