CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



767 



(Usually two hours if sodium phos- 

 phate is used and longer if Na2C03-) 



(6) Filter thru cotton, using compressed 

 air to effect a fast filtration. 



(7) Distribute into small sterile flasks. 



(8) Finally distribute sterile (7) into 

 sterile test tubes that have been 

 autoclaved for 2J hours. (The tubes 

 are contained in an enamel container 

 in the autoclave. The inner tem- 

 perature reaching about 150°C.) 



Sterilization: Heat in streaming vapor for 



two hours. 

 Use: General culture medium. 

 Reference: Jacobi (1888 p. 540). 



2381. Jacobi's Infusion Bjoth Fucus 

 Medium 



Constituents : 



1. Meat infusion 1500.0 cc. 



2. Peptone (siccum) 15.0 g. 



3. NaCl 7.5 g. 



4. Fucus 37.5 g. 



Preparation: 



(1) Method of preparation of meat 

 infusion not given. 



(2) Add 2, 3 and 4 to (1). 



(3) Heat over a free flame until the 

 materials are dissolved. 



(4) Make up to the original volume by 

 addition of water. 



(5) Press thru a towel for some of the 

 Fucus will not dissolve. 



(6) Add Na2C03 or sodium phosphate 

 until the reaction is slightly alkaline. 



(7) Pour into a flask and steam until the 

 albuminous material still to be 

 removed is completely separated. 

 (Usually 2 hours if sodium phosphate 

 is used and longer if Na2C03.) 



(8) Filter thru cotton, using compressed 

 air to effect a fast filtration. 



(9) Distribute into smaller sterile flasks. 

 (10) Finally distribute sterile (9) into 



sterile test tubes that have been 

 autoclaved in the autoclave for 2^ 

 hours. (The tubes are contained 

 in an enamel container in the auto- 

 clave. The inner temperature reach- 

 ing about 150°C.) 



Sterilization: Heat (9) in streaming vapor 

 for two hours. 



Use: General culture medium. 



Reference: Jacobi (1888 p. 540). 



2382. Celli's Bouillon Fucus Medium 



Constituents: 



1. Bouillon 1000.0 cc. 



2. Fucus crispus (5.0%) 50.0 g. 



Preparation : 



(1) Prepare a 5.0% solution of Fucus 

 crispus in bouillon. (Author also 

 used water instead of bouillon.) 



(2) Add for each 10.0 cc. medium, 1.0 cc. 

 of N/10 NaOH solution or 4 to 5.0 cc. 

 saturated solution of Na2C0j. 



(3) Distribute in plates. 

 Sterilization: Method not given. 



Use: Isolation and cultivation of amoeba. 

 Reference: Celli (1896 p. 537). 



