GROUP V. INITIALLY LIQUID MEDIA BECOMING PERMA- 

 NENTLY SOLID. SOLIDIFYING AGENT ORGANIC 



Key to the Subgroups of Group V 2383. Matzuschita's Basal Rice Flour 



Ai. Solidifying agent of plant origin. Medium 



Subgroup V-A (Med. 2383 to 2397) Constituents : 



A2. Solidifying agent of animal origin. 1- Water 1000.0 cc 



Bi. Solidified by blood or its derivatives. 2. Rice flour 



Subgroup V-B (Med. 2398 to 2430) Preparation : 



B2. Solidified by materials other than blood (1) Boil water containing rice flour 



or its derivatives (eggs, etc.) (amount not given). 



Subgroup V-C (Med. 2431 to 2465) (2) To the paste formed, either add 2.0% 



glucose or 6.0% glycerol or use plain 



SUBGROUP V-A without any additions. 



Sterilization: Sterilize in the autoclave. 



Solidifying Agent of Plant Origin ^'11^.^!')^'°! , "^.,,.^7°:^"^^ ^^d 



T> 1 >. 1 .L -R/r J- ^ . chicken tubercle bacilli. Author reported 

 Basal or Complete Media Contain- that best growth of mammalian type on 

 ing Materials of Plant Origin, glycerol medium. Its colonies were 

 Initially Liquid but Becom- orange yellow. Chicken type gave whit- 

 ing Permanently Solid ^^^ S!'^^' grayish black or yellowish 



colonies. 



Ai. Additional materials, if any, of known Added nutrients: The author added 2.0% 



chemical composition. glucose or 6.0% glycerol. 



Matzuschita's Basal Rice Flour Variants: 



Medium 2383 (a) The author used the basal medium 



Hefferan's Starch Medium 2384 without any additions. 



Lloyd, Clark and McCrae's Gluten (b) Buchanan cultivated Monascus pur- 

 Medium 2385 purens from silage on a medium 



Baginsky's Basal Starch Medium. . . 2386 prepared from a thick paste prepared 



Uschinsky's Asparaginate Starch from rice flour and tap water. 



Medium (Smith) 2387 References : Matzuschita (1899 p. 128), 



Hefferan's Peptone Starch Medium. 2388 Buchanan (1910 p. 103). 



Kita's Bran Medium 2389 



Kita's Rice Medium 2390 2384. Hefferan's Starch Medium 



Kita's Soy Bean Medium 2391 Constituents : 



As. At least one additional constituent of 1. Water... innn n ^« 



1 -1 ., ., i-UUU.u cc. 



unknown chemical composition. 2. Starch (10.0%) 100 g 



Bi. Peptone present. Preparation : 



Hefferan's Peptone Rice Flour d) Cook 10.0% starch with water 



Medium 2392 (2) Pour into petri dishes. 



Lloyd, Clark and McCrea's Bouillon Sterilization : Sterilize by the discontinuous 



Corn Flour Medium 2393 method in the steamer 



Lohnis' Bouillon Milk Rice Medium . 2394 Use : Cultivation of red pigment producing 



B2. Peptone not present. organisms, B. prodigiosus, B. ruber bal- 



Sanguineti's Yeast Extract Starch ticus, B. kilienis, B. ruber miquel, B. 



M®d^^°^ 2395 rutilus, B. amyloruber. Author reported 



Harras' Tissue Starch Medium 2396 growth. Good growth was obtained 



Smith's Milk Rice Medium 2397 when 1.0% peptone was added. 



768 



