770 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(9) Allow the starch to stand in the 

 ice box for about a week in distilled 

 water (3 liters per jar or beaker). 

 Siphon off the water twice a day at 

 first, and afterwards once a day, 

 stirring up the starch thoroughly 

 each time fresh water is added. 



(10) Drain the starch very free from 

 water, scoop up with sterile spoons 

 or spatulas and place in sterile 

 petri dishes. 



(11) Dry in the blood serum oven at 

 56°C., the cover being raised on 

 corks an inch to let the moisture 

 out. 



(b) Smith prepared a similar medium as 

 follows : 



(1) Dissolve 30.0 g. to 40.0 g. glycerol, 

 5.0 to 7.0 g. NaCl, 0.1 g. CaCl., 

 0.3 to 0.4 g. MgS04, 2.0 to 2.5 g. 

 K2HPO4, 6.0 to 7.0 g. ammonium 

 lactate, 3.0 to 4.0 g. sodium 

 asparaginate in 1000.0 cc. of water. 

 The glycerol may be omitted. 



(2) Add 1.0 g. of potato starch to each 

 10.0 cc. of (1) in a test tube, and 

 proceed as above. 



References: Smith (1899 p. 102), (1905 p. 

 196), Tanner (1919 p. 61). 



2388. Hefferan's Peptone Starch Medium 



Same as medium 26 with the addition of 

 1.0% peptone. 



2389. Kita's Bran Medium 

 Constituents : 



1. Water. 



2. Wheat bran. 

 Preparation : 



(1) Add 25.0 g. wheat bran to 10.0 g. water 

 or 10.0% wheat bran to 20.0 g. water. 

 Details of preparation not given. 



(2) Place in Erlenmeyer flasks. 

 Sterilization: Not specified. 



Use: Cultivation of Japanese molds, As- 

 pergillus Okazaki, Aspergillus candidus, 

 Aspergillus albus, Aspergillus tamarii 

 Pseudorhizopus, Aspergillus glaucus and 

 other molds. 



Variants : 



(a) The author used rice bran instead of 

 wheat bran. 



(b) The author steam.ed 10.0 g. of rice 



bran with 10.0 g. of water in a 400.0 

 cc. Erlenmeyer flask. 

 Reference: Kita (1913 p. 446), (1914 p. 354). 



2390. Kita's Rice Medium 

 Constituents : 



1. Water. 



2. Rice. 

 Preparation : 



(1) Prepare one of the following com- 

 binations: 



(a) 100.0 g. rice + 100.0 g. water. 



(b) 25.0 g. rice + 10.0 cc. water. 



(c) 10.0 g. rice + 5.0 cc. water. 



(d) 100.0 g. rice + 50.0 cc. water. 



(2) Place in Erlenmeyer flasks. 

 Sterilization: Not specified. 



Use: Cultivation of Japanese molds, As- 

 pergillus Okazaki, Aspergillus candidus, 

 Aspergillus albus, Aspergillus tamarii 

 Pseudorhizopus Aspergillus glaucus and 

 other molds. 



Variants : The author steamed 25.0 g. of rice 

 with 15.0 cc. of water in a 400.0 cc. Erlen- 

 meyer flask. 



Reference: Kita (1913 p. 446), (1914 p. 353). 



2391. Kita's Soy Bean Medium 



Same as medium 2390 but the author used 

 fat-free soy bean flour instead of rice. 

 Reference: Kita (1914 p. 356), (1913 p. 446). 



2392. Hefferan's Peptone Rice Flour 

 Medium 

 Constituents : 



1- Water 100.0 cc. 



2. Rice flour 35 q g. 



3. Peptone 1 q g' 



Preparation : 



(1) Cook 35.0% rice flour and 1.0% pep- 

 tone with water. 



(2) Pour into petri dishes. 

 Sterilization : Sterilize by the discontinuous 



method. 

 Use: Cultivation of red pigment producing 



bacteria B. prodigiosus, B. ruber bal- 



ticus, B. kilienis, B. ruber miquel, B. 



rutilus, B. amyloruber. 

 Reference: Hefferan (1903-04 p. 520). 



2393. Lloyd, Clark and McCrea's Bouillon 



Corn Flour Medium 

 Constituents : 



1. Bouillon 1000.0 cc. 



2. Corn flour starch 70.0 g. 



