CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



771 



Preparation : 



(1) Exact composition of broth not given. 



(2) Neutralize (1) using phenol red as an 

 indicator. 



(3) Add starch to (2). 



(4) Shake well. 



(5) Tube. 



(6) Gelatinize by boiling, shaking until 

 gelatinization is complete. 



Sterilization: Sterilize in the autoclave at 



20 minutes at 115°C. 

 Use: Cultivation of Fliigge's Mesentericus 



group. Author especially recommended 



this medium for stab cultures. 

 Reference: Lloyd, Clark and McCrea 



(1920-21 p. 383). 



2394. Lohnis' Bouillon Milk Rice Medium 



Constituents: 



1. Bouillon 50.0 cc. 



2. Rice meal 100.0 g. 



3. Milk 150.0 cc. 



Preparation: (1) Mix 1, 2 and 3. 



Sterilization: Sterilize on each of three 



successive days. 

 Use : Cultivation of molds. 

 Variants : 



(a) Besson prepared the medium as 

 follows: 



(1) Thoroughly mix 100.0 g. of rice 

 powder with 50.0 cc. of peptone 

 bouillon and 150.0 cc. of milk. 



(2) Distribute in layers 1 to 2 centi- 

 meters thick in petri dishes. 



(3) Heat at 115°C. for 20 minutes. 

 Besson noted that this medium had 

 been described by Soyka. 



(b) Harvey prepared a medium as 

 follows: 



(1) Prepare a paste from 10.0 parts rice 

 flour, 7 parts infusion broth (see 779 

 (bb)) and 2 parts milk. 



(2) Distribute as a layer in test tubes 

 or flasks. 



(3) Heat over boiling water to solidify 

 the paste. 



(4) Sterilize 30 minutes at 100°C. on 

 3 days. 



(c) Harvey prepared a similar medium 



as follows: 

 (1) Prepare a paste from 2 parts rice 

 flour, 1 part infusion broth (see 

 779 (bb)) and 3 parts milk. 



(2) Distribute as a layer in test tubes 

 or flasks. 



(3) Sterilize 30 minutes at 100°C. on 3 

 days. 



(4) The paste may be distributed in 

 petri dishes and sterilized at 115°C. 

 for 20 minutes. 



References: Lohnis (1913 p. 88), Besson 

 (1920 p. 58), Tanner (1919 p. 72), Harvey 

 (1921-22 p. 95). 



2395. Sanguineti's Yeast Extract Starch 

 Medium 



Constituents : 



1. Yeast extract 500.0 cc. 



2. Starch 15.0 g. 



Preparation : 



(1) Preparation of yeast extract not 

 given. 



(2) Distribute yeast extract in 500.0 cc. 

 lots in 1500.0 cc. flasks. 



(3) Add 15.0 g. of starch to each flask. 



(4) Boil for 30 minutes on a salt water 

 bath. 



Sterilization: Autoclave for 30 minutes at 



120 °C. 

 Use: To study fermentation of starch by 



Aspergillus orizae, Mucor alternans, 



Aimjlomyces Rouxii. 

 Reference: Sanguineti (1897 p. 267). 



2396. Harrass' Tissue Starch Medium 



Constituents : 



1. Water. 



2. Liver or brain. 



3. Starch. 

 Preparation : 



(1) Pass liver or brain through a meat 

 grinder. 



(2) Prepare a paste with starch in the 

 usual manner. (Method not given.) 



(3) Mix (1) and (2) thoroughly (amounts 

 not specified). 



(4) Distribute in petri dishes. 

 Sterilization: Sterilize in streaming steam. 

 Use: Cultivate obligate anaerobes. Bac. 



butyricus, Bac. botulinus, the black leg 

 bacillus, the maligenens oedema bacillus, 

 and other obligate anaerobes. This me- 

 dium gave a quite solid medium enabling 

 one to obtain pure cultures. 

 Reference: Harrass (1906 p. 2339). 



