772 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2397. Smith's Milk Rice Medium 



Constituents: 



1. Milk 100.0 cc. 



2. Rice 10.0 to 20.0 g. 



Preparation: (1) Place one or two grams of 



rice into each tube containing 10.0 cc. of 

 milk. 



Sterilization: Not specified. 



Use: Cultivation of chromogenic plant 

 parasites. Klimmer cultivated skin mi- 

 crophytes on a similar medium. 



Variants: Klimmer prepared a similar 

 medium as follows: 



(1) Thoroughly mix 100.0 g. of rice 

 powder with 250.0 cc. of skimmed 

 cow's milk. 



(2) Heat in a porcelain dish, stirring 

 constantly. 



(3) Pour into cylindrical tubes. 



(4) When cool, shove the solidified rice 

 milk mixture from the tubes and cut 

 into dices. 



(5) Place the discs into petri dishes. 



(6) Add 8 drops of milk to each disc. 



(7) Sterilize for 1 to 1.5 hours. 

 Klimmer gave Krai the credit for describ- 

 ing this medium. 



References: Smith (1905 p. 48), Klimmer 

 (1923 p. 207). 



SUBGROUP V-B 



Basal or Complete Media Solidified 

 by Blood or Derivatives, Initially 

 Liquid but Becoming Per- 

 manently Solid 



Ai. Additional constituents, if any, of 



known chemical composition. 

 Bi. Additional organic constituents not 



employed. 



Frothingham's Coagulated Blood 

 Serum 2398 



Proco, et al., Gentian Violet Serum. 2399 



Lorrain Smith's Alkali Serum 

 (Harvey) 2400 



Duval's Trypsinized Serum 2401 



Carrel and Burrows' Plasma Me- 

 dium 2402 



Ball's Coagulated Blood Medium. . . 2403 

 B2. Additional organic constituents 



employed. 



Ci. Carbohydrates present. 

 Cobbetts' Glucose Serum Medium.. 2404 

 Costa's et al. Litmus Glucose 



Serum 2405 



Rankin's Potassium-Sulphocyanide 



Glucose Serum 2406 



Dubois' Glycerol Glucose Serum.... 2407 

 C2. Carbohydrates not present. 



Marx's Glycerol Ragit Serum 2408 



Picker's Glycerol Phosphate Serum. 2409 



Picker's Citrate Glycerol Serum 2410 



Proca, et al. Pyrogallic Acid Serum. 2411 



Shmamine's Nucleate Serum 2412 



A2. At least one additional constituent of 



unknown chemical composition. 

 Bi. Carbohydrates added. 

 Ci. Additional organic carbon of known 

 chemical composition added. 

 Conradi and Troch's Extract Broth 



Serum Medium 2413 



Hall and Stone's Glycerol Serum 



Medium 2414 



Greenspon's Veal Infusion Serum 



(Stitt) 2415 



Bacto Loeffler's Blood Serum 



(Dehydrated) 2416 



Loeffler's Glucose Infusion Serum . . 2417 

 Rankin's Cyanide Glucose Serum... 2418 

 Ball's Glucose Bouillon Blood Al- 

 bumin Medium 2419 



Crowe's Glucose Agar Blood 



Medium 2420 



C2. Additional organic carbon of known 



chemical composition not supplied. 

 B2. Carbohydrates not added. 

 Yardon's Bouillon Serum (De- 

 siccated) 2421 



Thalmann's Infusion Broth Serum 



Medium 2422 



Picker's Brain Infusion Serum 



Medium 2423 



Picker's Potato Juice Serum 



Medium 2424 



Vanned' s Nutrose Serum Medium. . 2425 

 Pergola's Egg Yolk Serum Medium. 2426 

 Greenspon's Glucose Citrate Serum 



Medium 2427 



Duval's Egg Serum Medium 2428 



Eberling's Serum Tissue Plasma 



Medium 2429 



Wolbach and Schesinger's Tissue 



Plasma Medium 2430 



