CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



779 



as good on this medium as on Loeffler's 

 serum. 

 Reference: Marx (1913 p. 250). 



2409. Picker's Glycerol Phosphate Serum 



Constituents : 



1. Blood serum 1000.0 cc 



2. KHoPO^ ^OS- 



3. Glycerol 20.0 g. 



Preparation: 



(1) Add 0.5% KH2PO4 to blood serum. 



(2) Add 2.0% glycerol to (1). 



(3) Solidify (method not given). 

 Sterilization: Method not given. 



Use: Cultivation of tubercle bacilli. 

 Author reported that the tubercle bacilli 

 grew without the glycerol. In concentra- 

 tion higher than 2.0% glycerol checked 

 the growth. 



Variants : 



(a) Thoinot and Masselin prepared a 

 similar medium as follows : 



(1) Autoclave glycerol at 115°C. 



(2) Add 6 to 8 parts by weight of (1) to 



100 parts of sterile serum. 



(3) Mix well. 



(4) Tube. 



(5) Solidify at a temperature between 

 75 to 86°C. 



(b) Harvey prepared a similar mediiim by 

 adding 7 parts sterile glycerol to 100 

 parts sterile ox serum, tubing and 

 coagulating in the steamer or inspis- 

 sator at 65° to 90°C. 



(c) Harvey gave the following method of 

 preparation: 



(1) Mix 5 parts glycerol and 95 parts ox 

 serum. 



(2) Heat 30 minutes at 56°C. in a water 

 bath on each of two successive 

 days. 



(3) Coagulate in the inspissator or 

 steamer at 65° to 90°C. 



(4) Test sterility before use by in- 

 cubating 48 hours. 



References: Ficker (1900 p. 510), Thoinot 

 and Masselin (1902 p. 48), Roux and 

 Rochaix (1911 p. 128), Besson (1920 p. 

 53), Dopter and Sacquepee (1921 p. 139), 

 Harvey (1921-22 pp. 78, 79). 

 2410. Picker's Citrate Glycerol Serum 



Constituents : 



1. Distilled water. 



20.0 cc. 



2. Blood serum 80-0 cc 



3. Magnesium citrate 0.25 g 



4. (NH4)2SO. 0.3 g 



5 Potassium phosphate 0.5 g 



6. MgS04 0-25 g 



7. Glycerol 2.0 g 



Preparation : 



(1) Dissolve 3, 4, 5, 6 and 7 in 1, 



(2) Add 80.0 cc. of serum. 



(3) Solidify (method not given). 

 Sterilization: Method not given. 

 Use: Cultivation of tubercle bacilli. 

 Variants: The author omitted the MgS04, 



used 0.2 g. (NH4)2S04 instead of 0.3 g. 

 and added 0.6 g. mannitol. 

 Reference: Ficker (1900 p. 510). 



2411. Proca, et al., Pyrogallic Acid Serum 



Constituents : 



1. Distilled water. 



2. Serum. 



3. Pyrogallic acid. 

 Preparation : 



(1) Dissolve 1.0 g. pyrogallic acid and 

 2.0 g. NaOH in 100.0 cc. distilled 

 water. 



(2) To 10.0 cc. serum (beef or horse) add 

 l.Occ. of (1). 



(3) Coagulate at 80°. 



Sterilization: Sterilize the serum in the 

 steamer using Tyndall's method. 



Use: Cultivation of syphilus spirochetes. 



Variants: Harvey specified that the pyro- 

 gallic acid solution be stored several 

 weeks before use, and inoculated the 

 solidified medium by passing a pipette 

 containing a culture material between the 

 medium and the wall of the tubes, seal 

 the tubes hermetically and then incubate. 



References : Proca, Danila and Stroe (1912 

 p. 895), Harvey (1921-22 p. 80). 



2412. Shmamine's Nucleate Serum 



Constituents : 



1. Serum ■• 200.0 cc. 



2 Sodium salt of nucleic 



■ acid 1.0tol.5g. 



Preparation : 



(1) Obtain horse blood under aseptic 

 conditions if possible. 



(2) Draw off the clear serum by means of 

 sterile pipettes. 



(3) Allow to stand in the ice box for two 

 or three days. 



