CCLTOEE MEDIA FOB CULTIVATION OF MICEOOEGANISMS 781 



2415 Greenspon's Veal Infusion Serum minutes following coagulation is rec- 



(<zr,u\ ommeneded. 



^"^ ^ Use: Cultivation of diphtheria bacilli. 



Constituents: Reference: Digestive Ferments Co. (1925 



1 Glucose veal infusion broth. . 24.0 cc. ^ ^^^ 



2. Sodium citrate (50.0% soln.) . 1.0 cc. " 



3 Serum, pig, sheep or human.. 75.0 cc. 2417. Loeffler's Glucose Infusion Blood 



^ ■ .. Serum 

 Preparation : 



(1) Add 1.0 cc. of a 50.0% sodium citrate Constituents: 



solution to 75.0 cc. of clear pig, sheep ^ g^^^^ ^^^^.g^) 300.0 cc. 



or human serum and sufficient glucose ^ Meat infusion 100.0 cc. 



veal infusion broth, or glucose meat ^ Peptone 1-0 S- 



extract broth to bring the volume to ^ ^^^^ 0.5 g. 



100.0 cc. 5. Dextrose 10 g. 



(2) Adjust the reaction to pH = 6.4 by preparation: 



the addition of 3.0% citric acid ^^^ Prepare meat infusion in the usual 



solution using di-brom-thymol blue manner (exact method not given). ^ 



as an indicator. (2) Dissolve 1.0 g. peptone, 0.5 g. NaCl 



(3) Tube. _ and 1.0 g. glucose in 100.0 cc. (1) 



(4) Coagulate in an inspissator. ^ making a bouillon. 

 Sterilization: Sterilize by the fractional ^^^ Obtain horse serum under sterile 



method on 3 successive days. conditions. 



Use: Cultivation of diphtheria bacilli. ^^^ ^^^.^ 3 p^^.^^ (3) ^^^^ ^ne part sterile 



The author reported that the citric acid ^2). 



inhibited the growth of organisms from ^^^ Solidify in Koch's apparatus, heating 



the mouth. to 90°C. 



Reference: Stitt (1923 p. 41). Sterilization: Not specified. 



Use : Cultivation of diphtheria bacilli. 



2416. Bacto Loeffler's Blood Serum Variants : The following authors have sug- 



(Dehydrated) gested the following methods of prep- 



^ i-i *„. aration. 



Constituents: hel (1896) 



1. Distilled water ^''\l)'prepare m;at infusion in the usual 



2. Serum, beef blood. _ 300.0 cc ( P^^^ ^^^^^^ ^^^ ^.^^^^^ 



3. Glucose infusion broth 100.0 cc. ^.^^^^^^ ^^ ^ ^^^^^^^^ ^^ ^ 



Preparation: . „ . t m ' NaCl and 10 g. glucose in 100.0 



(^^) Dissolve 80 g. of Bacto Loeffler's JNa^^^ anu i.u g. g, 



SI! re\ttl?in'r:,anteci position (4, ^ t^ts <3, wit. one pa. 



<4, Sot rilTaTand t.e door of the (5) Solidify in Koch's apparatus, 



autoclave before turning on the ,,, , ^-ff/X 



steam. Run the pressure to 15 O) Smr^^h (WO^ „„^ 



pounds as quickly as possible and (1) Preparation 



::"sur'e°^r°20°^.::;les"o:to 5 llZt (2, i'dl"l.O of glucose to (I,, 



rrrsreforeo minute. Bo not use C3, -- -fe ^ u^dr alt: 



over 15 pounds pressure. j-^ 



Stprilization- When coagulation is com- conditions ■ ^. ^ 



;;:t open le lowest'part and replace (4) Coagulate in the inspissator. 



the entrapped air with steam, maintaining (0 f-^t 903^ ^^^_ 



constant pressure during the change. ^^^ , % !f .hlttoir 



Sterilization at 15 pounds pressure for 20 tamer at an abattoir. 



