CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



785 



Sterilization: Not specified. 



Use: Cultivation of diphtheria bacilli. 



Variants: The serum may be substituted 



by a solution of dessicated serum. 

 Reference: Vardon (1923-24 p. 431). 



2422. Thalmann's Infusion Broth Serum 

 Medium 



Constituents : 



1. Distilled water 2000.0 cc. 



2. Beef 1000.0 cc. 



3. Peptone (siccum) 20.0 g. 



4. NaCl 10.0 g. 



5. Serum (beef or hog) 

 Preparation : 



(1) Cut lean beef into small pieces in a 

 meat cutting machine. 



(2) Add a double weight of distilled 

 water to (1). 



(3) Boil for i hour, stirring continually 

 with a glass rod. 



(4) Make up the loss of water and filter 

 thru a filtering cloth. 



(5) Add 1.0% peptone (siccum), and 

 0.5% NaCl. 



(6) Boil. 



(7) Make up the volume of water lost. 



(8) Cool (in a closed container) and 

 filter. 



(9) Distribute in 300 to 500.0 cc. portions 

 in clean flasks with patented sealers. 



(10) Sterilize in streaming steam for one 

 hour. 



(11) Bring to boil on a concentrated salt 

 solution bath and boil for 45 minutes 

 shaking often. 



(12) Take 30.0 cc. of (11), add a drop of 

 alcoholic phenolphthalein solution 

 and add N/1 sodium solution until a 

 red coloration is formed. 



(13) Estimate the amount of (11) and 

 calculating from (12) add I to f the 

 amount of sodium solution required 

 for neutralization. 



(14) Place in warm water. 



(15) Filter. 



(16) Mi.x equal portions of serum (beef or 

 hog, but hog serum preferred) and 

 sterile (15). 



(17) Tube. 



(18) Place in a serum oven in a slanted 

 position and heat on the first and 

 second day for 2 hours at 70°C. and 

 on the third day for one hour at 

 100 °C. 



Sterilization: Sterilization effected during 

 preparation. 



Use: Isolation of the gonococcus. The 

 author reported that after 16 hours, 

 gonococci colonies were round, dull 

 typical colonies. The addition of sugar 

 did not increase growth. 



Variants : Klimmer mixed one part bouillon 

 with three parts serum and coagulated at 

 65 to 80 °C. 



References: Thalmann (1900 p. 831), Klim- 

 mer (1923 p. 200). 



2423. Picker's Brain Infusion Serum 

 Medium 



Constituents: 



1. Distilled water 500.0 cc. 



2. Brain 500.0 g. 



3. Serum 500.0 cc. 



4. Glycerol 30.0 cc. 



Preparation : 



(1) Pass fresh brain (500.0 g.) thru a meat 

 chopping machine. 



(2) Add an equal weight of (500.0 g.) 

 distilled water. 



(3) Stir and heat slowly to a boil. 



(4) Boil slowly for i hour. 



(5) Filter thru a filtering cloth until the 

 filtrate assumes a pulpy character. 

 Press the coagulated mass free from 

 liquid. 



(6) Mix equal parts of sterile (5) and 

 serum. 



(7) Add 3.0% glycerol. 



(8) Tube. 



(9) Solidify in a serum oven. 

 Sterilization: Steam the filtrate from (5) 



for two hours. Sterilization of other 

 materials not specified. 



Use: Cultivation of tubercle bacilli. 

 Author reported that beef lungs, testicles, 

 spleen, liver, kidney, udder, pancreas 

 were treated in like manner but none 

 gave as good results as brain. The lungs, 

 spleen, liver and pancreas of man were 

 used, but brain proved to be the best 

 medium. 



Variants: Klimmer prepared the medium 

 as follows: 



(1) Cut fresh brain into small pieces and 

 add an equal weight of distilled water. 



(2) Heat to boiling, stirring constantly. 



(3) Filter thru a filtering cloth, and 

 squeeze the meat free from juice. 



