786 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(4) Sterilize for two hours (temperature 

 not given). 



(5) Add an equal volume of sterile solu- 

 tion of 3.0% glycerol in serum to (4) 

 under aseptic conditions. 



(6) Mix thoroly. 



(7) Solidify quickly. 



References: Ticker (1900 p. 593), Harvey 

 (1921-22 p. 98), Klimmer (1923 p. 224). 



2424. Picker's Potato Juice Serum Medium 



Constituents : 



1. Potato juice 100.0 cc. 



2. Serum (beef) 200.0 cc. 



3. Glycerol (3.0%) 6.0 cc. 



Preparation : 



(1) Exact method of preparation of 

 potato juice not given. 



(2) Mix one part (1) with two parts beef 

 serum. 



(3) Add 3.0% glycerol. 



(4) Reaction may be neutral or slightly 

 alkaline or acid. 



(5) Solidify in a serum oven. 

 Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. Au- 

 thor reported that best growth was ob- 

 tained on neutral or slightly alkaline 

 medium. 



Reference: Ficker (1900 p. 509). 



2425. Vannod's Nutrose Serum Medium 



Constituents : 



1. Distilled water 40.0 to 50.0 cc. 



2. Serum (hog) 15.0 cc. 



3. Glycerol 3.0 cc. 



4. Nutrose 1 .0 cc. 



Preparation : 



(1) Obtain hog blood from the abattoir. 



(2) After 24 hours syphon the serum 

 from the blood cells. 



(3) Filter the serum thru a Berkefeld 

 candle to free it from red corpuscles. 



(4) Add 40, 45 or 50.0 cc. distilled water 

 to 15.0 cc. of the filtered serum and 

 mix thoroly. 



(5) Add 3.0 cc. of glycerol and 1.0 cc. of 

 nutrose to each flask of (4). 



(6) Heat by a flame, and shake until 

 boiling. 



Sterilization: Sterilize three successive 



times in steam at 102°C. 

 Use: Cultivation of gonococci. 

 Reference: Vannod (1907 p. 13). 



2426. Pergola's Egg Yolk Serum Medium 

 Constituents: 



1. Ox serum 50.0 cc. 



2. NaCl (0.8% soln.) 50.0 cc. 



3. Potassium tellurite (1.0% 



soln.) 2.0 cc. 



4. Egg yolk 1 



Preparation: Details of preparation not 



given in the abstract. 

 Sterilization: Not given. 

 Use: Diagnosis of diphtheria. 

 Reference: Pergola (1918 p. 101 taken 



from 1919 p. 57). 



2427. Greenspon's Glucose Citrate Serum 



Medium 



Constituents : 



1. Glucose veal infusion broth 



to 100.0 cc. 



2. Serum, pig, sheep or human . . 75.0 cc. 



3. Sodium citrate (50.0% soln.) . 1.0 cc. 

 Preparation : 



(1) Add 1.0 cc. of a 50.0% sodium citrate 

 solution to 75.0 cc. of clear pig, sheep 

 or human serum and sufficient glucose 

 veal infusion broth to bring the 

 volume to 100.0 cc. 



(2) Adjust to pH 6.4 by the addition of 

 3.0% citric acid. 



(3) Tube. 



(4) Coagulate in the Koch inspissator. 

 Sterilization: Sterilize the fractional 



method on each of three successive days. 



Use: Isolation and cultivation of diph- 

 theria bacillus. 



Variants: Beef extract broth may be used 

 instead of veal infusion. 



Reference: Greenspon (1922 p. 32). 



2428. Duval's Egg Serum Medium 



Constituents : 



1. Distilled water 200.0 cc. 



2. Human blood 100.0 cc. 



3. Glycerol 10.0 cc. 



4. Eggs 12 



Preparation : 



(1) Mix 200.0 cc. distilled water, 100.0 

 cc. of human blood serum and 10.0 

 cc. of glycerol. 



(2) Carefully separate the whites of 12 

 eggs from the yolks and drop each 

 separately and aseptically into a 

 flask containing sterile (1). 



