788 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(Authors have kept tissue cultures from 

 the testicles and brain of guinea pigs in 

 active growth for four to six generations 

 over a period of four to six weeks). Use 

 guinea pig brain for growing typhus 

 organism and guinea pig testicles for the 

 cultivation of Rocky Mountain Spotted 

 Fever microorganism. For injection into 

 animals the tissue cultures are removed 

 from the coverslips, placed in Ringer's 

 solution and teased into pieces small 

 enough to pass thru a number 18-gauge 

 hypodermic needle. 

 Reference: Wolbach and Schlesinger (1923, 

 p. 233). 



SUBGROUP V-C 



Solidifying Agent Egg or Egg 

 Derivatives, etc. 



Basal or Complete Media Solidified by 

 Materials other than Blood or its Deriv- 

 atives (Egg or Egg Derivatives, 

 etc.) Initially Liquid but Becom- 

 ing Permanently Solid 



Ai. Solidified by whole egg. 

 Bi. Additional constituents, if any, of 

 known chemical composition. 

 Dal Pozzo's Lapwing Egg Medium . 2431 



Ball's Boiled Egg Medium 2432 



Abel's Egg Medium 2433 



Smith's Egg Medium 2434 



Dorset's Egg Medium (Heinemann) 2435 

 Modified Dorset's Egg Medium 



(Brown and Smith) 2436 



Dorset's Egg Medium (Abel) 2437 



Soparkar's Glycerol Egg Medium... 2438 

 Lubenau's Glycerol Egg Medium 



(Abel) 2439 



Twort and Ingram's Basal Glycerol 



Egg Medium 2440 



Bj. At least one additional constituent of 

 unknown chemical composition present. 

 Roddy's Glucose Bouillon Egg 



Medium 2441 



Putnam and Gay's Milk Egg 



Medium 2442 



Petroff's Gentian Violet Egg 



Medium 2443 



Harvey's Sperm Egg Medium 2444 



Harvey's Olive Oil Egg Medium 2445 



Lubenau's Glycerol Bouillon Egg 



Medium (Park and Krumwiede) . . . 2446 



Corper's, et al.. Gentian Violet Egg 

 Medium 2447 



Twort's Bacteria Infusion Egg 



Medium 2448 



Harvey's Trypsinized Heart Egg 

 Medium 2449 



Rosenthal and Schulz's Meat In- 

 fusion Egg Medium (Zimmer- 



mann) 2450 



A2. Solidified by egg white. 



Thoinot and Masselin's Egg Albumin 

 Medium 2451 



Barthel's Egg Albumin Medium 2452 



Dal Pozzo's Transparent Egg 

 Albumin 2453 



Rosenthal and Schulz's Alkaline Egg 

 Albumin (Zimmermann) 2454 



Barthel's Milk Egg Albumin 

 Medium 2455 



Brown and Orcutt's Veal Infusion 



Egg Albumin Medium 2456 



A3. Solidified by yolk of egg. 



Pergola's Tellurite Egg Yolk 

 Medium 2457 



Smith's Egg Yolk Medium 2458 



McCoy and Chapin's Egg Yolk 

 (Francis) 2459 



Dorset's Egg Yolk Medium (Heine- 

 mann) 2460 



Nastinkoff's Egg Yolk Medium 

 (Rechtsamer) 2461 



Dal Pozzo's Egg Yolk Medium 2462 



Lubenau's Bouillon Egg Yolk 

 Medium (Schoenburg) 2463 



Phisalix's Potato Egg Yolk Medium 2464 

 A4. Solidified by other materials. 



Steffen's Sputum Medium 2465 



McCann's Ovarian Cyst Fluid 

 Medium 2466 



2431. Dal Pozzo's Lapwing Egg Medium 



Constituents : 



1. Water 1000.0 cc. 



2. Egg (lapwing) 100.0 cc. 



Preparation : 



(1) Wash lapwing's eggs with a 1% sub- 

 limate solution. (One egg will make 

 about 4 or 5 tubes of medium.) 



(2) Open the egg under aseptic conditions 

 and allow the white of the egg to flow 

 into a sterile flask. 



(3) Mix the egg albumin with its volume 

 of sterile water. 



(4) Distribute into sterile test tubes. 



