CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



789 



(5) Coagulate in the same manner as the 



coagulation of serum, (details of 



method not given) at a temperature 



of about 70°C. 



Sterilization: Method of sterilization of 



water or containers not given. 

 Use: Cultivation of saprophytic and para- 

 sitic forms. 

 Variants : The author added a sterile solu- 

 tion of glycerol, dextrin, or any other 

 carbohydrate to the medium. 

 Reference: Dal Pozzo (1888 p. 526). 



2432. Ball's Boiled Egg Medium 



Constituents : 



1. Egg. 

 Preparation : 



(1) Boil eggs. 



(2) Remove the shell over a small portion. 

 Sterilization: Not specified. 



Use: General culture medium. The co- 

 agulated albumin was stroked with the 

 inoculum. 



Variants: Various authors have described 

 similar media, and prepared them as 

 follows: 



(a) Bezangon. 



(1) Boil an egg. 



(2) Remove the shell. 



(3) Cut into discs. 



(4) Place the discs in Petri dishes. 



(5) Sterilization not specified. 



(b) Besson. 



(1) Boil an egg. 



(2) Remove the shell. 



(3) Cut the egg into thin discs and place 

 in Petri dishes. 



(4) Heat at 115°C. to sterilize. 



(c) Harvey. 



(1) Place whole eggs in 10 N sodium 

 hydroxide for 10 days. 



(2) Remove the shells. 



(3) Cut the eggs into fine slices. 



(4) Wash for 2 hours in running water. 



(5) Steam 60 minutes. 



(6) Transfer each slice of egg by means 

 of sterile forceps to a sterile Petri 

 dish. 



(7) Sterilize 20 minutes at 100°C. 3 

 days. 



(d) Klimmer. 



(1) Boil eggs. 



(2) Cut into strips or prisms. 



(3) Add several drops of sterile water 

 to each piece. 



(4) Sterilize (method not given). 

 References: Ball (1919 p. 84), Bezangon 



(1920 p. 121), Besson (1920 p. 54), Harvey 

 (1921-22 p. 85), Klimmer (1923 p. 223). 



2433. Abel's Egg Medium 



Constituents : 



1. Egg. 

 Preparation : 



(1) Carefully wash and brush the shell 

 of an egg. 



(2) Wash in a 5.0% sublimate solution 

 and in sterile water. 



(3) Dry with sterile wool. 



(4) Make a small opening in the apex of 

 the egg. 



(5) After inoculation close the opening 

 with sealing wax or sterile paper and 

 collodion. 



Use: General culture medium. 

 Variants : 



(a) Roddy prepared the medium as 

 follows: 



(1) Shake thoroly a fresh (not more 

 than 24 hours old) egg, having a 

 perfect shell with no crack. 



(2) Wash in warm sterile water, then 

 with warm 1:500 bichloride solution 

 and again with sterile water. 



(3) Wipe dry. 



(4) Inoculate by piercing the shell with 

 a sterile needle and introducing 

 the bacteria with a platinum loop. 



(5) Seal the opening with sterile sealing 

 wax. 



(6) Coat the egg with paraffin to obtain 

 anaerobic conditions. 



(b) Bezangon gave the following method 

 of preparation: 



(1) Sterilize the shell of an egg. 



(2) Make a small hole in the egg shell 

 near the region of the air chamber. 



(3) Inoculate. 



(4) Seal the opening with wax. 

 References: Abel (1912 p. 26), Roddy (1917 



p. 45), Bezangon (1920 p. 121). 



2434. Smith's Egg Medium 



Constituents : 

 1. Egg. 



