CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



799 



used) into sterile Petri dishes and 

 inspissated for 3 hours at 70°C. 



(2) An excised leprous nodule is then 

 cut into thin slices, 2 to 4 milli- 

 meters in breadth and 0.5 to 1.0 

 mm. in thickness and distributed 

 over the surface of the coagulated 

 albumin or serum. 



(3) Bathe the medium then seeded, 

 with a 1.0% sterile trypsin solution, 

 taking care not to submerge the 

 pieces of leprous tissue. Add suffi- 

 cient fluid to moisten thoroly the 

 surface of the medium. 



(4) Incubate in a moist chamber at 

 37°C. for a week to ten days. 

 Remove the plates from time to 

 time and add more trypsin as 

 evaporation necessitates. 



(c) Besson. 



(1) Sterilize the shell of an egg by 

 washing in a sublimate solution. 



(2) Dry with sterile filter paper. 



(3) Heat the end of the egg in a flame 

 until the shell is black. 



(4) Make a small hole in this end 

 using a flamed knife point. 



(5) Aspirate the white of the egg using 

 a sterile pipette. 



(6) Distribute in sterile tubes. 



(7) Coagulate by heating at 70°C. as 

 for the coagulation of serum. 



References : Thoinot and Masselin (1902 p. 

 53), Smith (1905 p. 48), Duval (1911 p. 

 369), Abel (1912 p. 27), Besson (1920 p. 

 54), Harvey (1921-22 p. 86). 



2452. Barthels Egg Albumin Medium 



Constituents : 



1. Water. 



2. Egg albumin. 

 Preparation: 



(1) Cut egg albumin in small cubes. 

 (Passini-Achalm's method, details 

 not given.) 



(2) Place in water in Gruber's tubes. 

 Sterilization: Sterilize for 15 minutes at 



125°C. 



Use: Cultivation of anaerobic bacteria 

 from milk. Author inoculated the tubes 

 with milk that had been fermented under 

 anaerobic conditions, and removed the 

 air from the tubes. 



Reference: Barthel (1910 p. 6). 



2453. Dal Pozzo's Transparent Egg Albumin 



Constituents : 



1. Water. 



2. Egg albumin. 

 Preparation : 



(1) Clean the eggs thoroly and open. 



(2) Catch the thin albuminous liquid 

 flowing out first in a sterile container. 



(3) Add i portion of water. 



(4) Distribute into test tubes. 



(5) Slant. 



Sterilization: Sterilize by the discontinuous 

 method in the steamer. 



Use: Cultivation of saprophytic and para- 

 sitic organisms. 



Variants: The author reported that gly- 

 cerol, dextrin, etc., might be added as 

 needed. 



Reference: Dal Pozzo (1888 p. 151). 



2454. Rosenthal and Schulz's Alkaline Egg 



Albumin (Zimmermann) 

 Constituents : 



1. Water. 



2. Egg albumin. 



3. KOH (1.0% solution). 

 Preparation : 



(1) Filter egg albumin thru a double 

 layer of muslin by hand under the 

 application of pressure. 



(2) Place the clear filtrate in a measuring 

 cylinder fitted with a ground glass 

 stopper. 



(3) Add to 5.0 cc. of albumin, 3.0 cc. of a 

 1.0% solution of KOH and 2.0 cc. of 

 water. 



(4) Allow to stand an hour, shaking only 

 back and forth, to mix the alkali, egg 

 and water. 



(5) Distribute into sterile test tubes or 

 other sterile culture containers. 



(6) Place the containers in hot water 

 (95°-98°) for a short time to coagulate 

 the albumin. 



Sterilization: Method not specified. 

 Use: General culture medium. 

 Variants : 



(a) Rosenthal and Schulz used NaOH 

 instead of KOH. 



(b) Salts such as NaCl, NasCOs, K2CO3, 

 NasSOi, Na2HP04, etc., may be 

 added. 



Reference: Rosenthal and Shulz (1888 p. 

 307), Zimmermann (1888 p. 314). 



