800 CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2455. Barthel's Milk Egg Albumin Medium Sterilization : Not specified. 



Constituents: Use: Cultivation of plant parasites. 



1- Water 1000.0 cc. Variants ; The whole egg may be boiled 



2. Glucose (1.0%) 10.0 g. ^^^^' ^^^ yolk removed and cut into 



3. Egg albumin pieces with a sterile knife and distributed 



4. Milk into sterile Petri dishes. 

 Preparation: Reference: Smith (1905 p. 49). 



(1) Add 0.1 cc. milk to 5.0 cc. of a 1.0% o^en -mt n j ^, 



glucose solution. " 2*^^' ^^^^^ f^^ ^hapin's Egg Yolk 



(2) Add (1) to Gruber's tubes containing (Francis) 

 sterile egg white. Constituents : 



Sterilization: Sterilize (2) at 125 to 130°C. ^- Saline solution (40.0%) 40.0 cc. 



(Method not given) . 2. Egg yolk (60.0%) 60.0 cc'. 



Use: Cultivation of anaerobic bacteria Preparation: 



from milk. (1) Thoroly wash fresh hen eggs in 



Reference: Barthel (1910 p. 6). soap, water and alcohol. Remove 



„._, _ ^ ^ . all the alcohol from the ees bv ie- 



2456. Brown and Orcutt's Veal Infusion Egg siting the alcohol 



Albumin Medium (2) Cut the egg in two using a sterile 



Constituents: sharp knife and separate the white 



1. Veal infusion broth 1000.0 cc. by decanting them from one half the 



2. Egg albumin 3000.0 cc. shell to the other, thus allowing the 



Preparation : whites to drain from the yolks. 



(1) Prepare standard veal infusion broth, (3) Collect the yolks in a sterile beaker. 



(2) Mix 3 parts egg albumin with 1 part (^) ^^d 40.0% sterile saline solution to 

 (1). 60.0% egg yolk. 



(3) Tube. (5) Mix thoroly. 



(4) Slant and coagulate in the inspissator. (6) Tube using a sterile funnel. 

 Sterilization: Not specified. (7) Place tubes in metal racks con- 

 Use: Cultivation of Bacillus pyogenes. structed so as to allow one-half inch 

 Reference: Brown and Orcutt (1920 p. space between the tubes for cir- 



224). culation. 



7SK'7 T)«^„^i».. x 11 •* T. V „ ^^^ ^^^* *^^ racked tubes in a slanting 



2457. Pergola^ Tellurite Egg Yolk position for the first half hour at 



^^'''"°' 70°C. and for the second half hour 



Constituents: at 72°C. 



1. Egg yolk.... 75.0 g. (9) Substitute sterile paraffined corks 



2. Glycerol 12.5 g. for the cotton plugs. 



3. NaCl (0.8% soln.) 12.5 cc. (10) Incubate the tubes in an upright 



4. Potassium tellurite 0.1 g. position for 3 or 4 days to test 



Preparation : Method not given in abstract. sterility. 



Sterilization: Method not given in abstract. Sterilization: Method of sterilization of 



Use: Cultivation of diphtheria bacilli. saline solution not given. 



Reference: Pergola (1925 p. 537) taken from Use: Cultivation of Bacterium tularense. 



(1925 p. 420). Reference: Francis (1922 p. 103). 



2458. Smith's Egg Yolk Medium 2460. Dorset's Egg Yolk Medium 



Constituents : (Heinemann) 



1. Egg yolk. Constituents: 



Preparation: 1. Water. 



(1) Remove the yolks from eggs under 2. Egg yolk, 

 aseptic conditions. Preparation: 



(2) Distribute into sterile test tubes. (1) Add 5.0 to 10.0 g. of sterile water to 



(3) Coagulate in a slanting position at the yolks of 3 or 4 eggs. 

 80°C. (2) Shake the flasks gently. 



