CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



801 



I (3) Tube in 10.0 cc. quantities. 



» (4) Heat in a Koch inspissator on 2 



successive days for 4 or 5 hours at 

 70°C. 

 Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Heinemann (1905 p 130). 



2461. NastinkofE's Egg Yolk Medium 

 (Reaktsamer) 



Constituents: 



1. Distilled water 600.0 cc. 



2. Egg Yolk 300.0 cc. 



3. NaOH (1.0%) 100.0 cc. 



Preparation : 



(1) To 300.0 cc. of egg yolk add 600.0 cc 

 of sterile distilled water and 100.0 

 cc. 1.0% sterile NaOH solution. 



(2) Distribute into test tubes and heat 

 on a water bath to 75-85°C. 



(3) Heat once more to 85°C. which is 

 sufficient to sterilize the coagulated 

 transparent medium. 



Sterilization: Method of sterilization not 



given. 

 Use: Cultivation of diphtheria, cholera, 



influenza and other pathogenic forms. 

 Reference: Nastinkoff (1893 j^33 and 34), 



Reaktsamer (1895 p. 492). 



2462. Dal Pozzo's Egg Yolk Medium 



Constituents : 



1. Water. 



2. Egg yolk. 

 Preparation : 



(1) Collect egg yolk from thoroly cleaned 

 eggs. 



(2) Add (1) to I volume sterile water 

 (may contain glycerol). 



(3) Filter. 



(4) Distribute into sterile test tubes. 



(5) Slant. 



Sterilization: Method of sterilization of 

 water not given. Sterilize the slants in 

 the steamer using the discontinuous 

 method. 



Use: Cultivation of saprophytic and para- 

 sitic organisms. 



Variants: Glycerol, dextrin, etc., may be 

 added as desired. 



Reference: Dal Pozzo (1887 p. 523). 



2463. Lubenau's Bouillon Egg Yolk Medium 

 (Schoenburg) 



Constituents : 



1. Bouillon. 



2. Glycerol. 



3. Egg yolk. 

 Preparation : 



(1) Wash eggs in hot soap solution. 



(2) Wash in alcohol and place in Petri 

 dishes. 



(3) Burn o£f the alcohol. 



(4) Make a small hole in the end of the 

 egg by means of a sterile glass knife. 



(5) Allow the egg white to run out. 



(6) Place the egg yolk in a sterile flask 

 containing small pieces of glass. 



(7) Shake well. 



(8) Add an equal volume of the ordinary 

 sterile filtered bouillon containing 

 3.0% glycerol and neutralized with 

 soda. 



(9) Mix thoroly. 



(10) Tube. 



(11) Solidify at 85 to 90°C. in a serum 

 apparatus by heating on 3 days for 



2 or 3 hours. 



Sterilization: Method of sterilization of 



(8) not given. 

 Use: Cultivation of tubercle bacilli. Other 



investigators, using glucose instead of 



glycerol, cultivated diphtheria bacilli. 

 Variants: Kolle and Wasserman prepared 



the medium as follows : 



(1) Thoroly wash eggs with hot soapy 

 water, place in alcohol for several 

 minutes and then burn the alcohol 

 from the eggs after they have been 

 removed. 



(2) Make a small opening in the egg shell 

 and allow the white to run out. 



(3) Remove the yolks and place in a 

 sterile flask. 



(4) Mix 100.0 cc. of neutral to litmus 

 meat infusion solution with the yolks 

 of 5 or 6 eggs. 



(5) Add 1.0% glucose (to cultivate 

 diphtheria bacilli) or 3.0%, glycerol 

 (to cultivate tubercle bacilli) to (4). 



(6) Solidify by heating at 90° on each of 



3 successive days for 2 or 3 hours. 

 References: Schoenburg (1911-12 p. 487), 



Kolle and Wassermann (1912 p. 414), 

 Besson (1920 p. 54), Harvey (1921-22 p. 



