802 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2464. Phisalix's Potato Egg Yolk Medium 

 Constituents: 



1. Potato pur66^ 



2. Glycerol. 



3. Egg Yolk. 

 Preparation : 



(1) Prepare a potato pure6 containing a 

 small amount of glycerol (method not 

 given). 



(2) Add egg yolk to (1) until a soft paste 

 is obtained. 



(3) Spread (2) in tubes or plates. 

 Sterilization: Sterilize in the autoclave. 



A solid mass forms. 



Use: Cultivation of tubercle bacilli. 

 Author reported that it may be necessary 

 to moisten the surface of the egg yolk 

 medium by the addition of sterile water 

 or glycerol bouillon. 



Reference: Phisalix (1903 p. 604). 



2465. Stefifen's Sputum Medium 



Constituents : 

 1. Sputum. 

 Preparation : 



(1) Remove all visible impurities from 

 human sputa. 



(2) Heat (temperature not given) and 

 remove the dirt floating on top. 

 (The addition of water not specified.) 



(3) Place in test tubes and plates and 

 solidify (Method not given). 



Sterilization: Not specified. 

 Use: Cultivation of pneumococci, strepto- 

 cocci, staphylococci and diphtheria bacilli. 

 Reference: Steffen (1895 p. 464). 



2466. McCann's Ovarian Cyst Fluid 

 Medium 

 Constituents : 



1. Ovarian cyst fluid. 

 Preparation : 



(1) Collect the fluid from ovarian cyst 

 during an abdominal operation by 

 means of a sterilized trocar under 

 aseptic conditions. Reject the first 

 portion of the fluid, and collect the 

 remainder in tall cylindrical sterile 

 glass jars. 



(2) The jars should be fitted with accu- 

 rately fitting glass stoppers. They 

 should be cleaned with a 1.0% mer- 

 curic chloride solution followed by 

 absolute alcohol. The stopper 

 should be smeared with vaseline 

 containing corrosive sublimate. 



(3) Allow the fluid to stand in the ice 

 box for 24 hours. 



(4) Pipette off the supernatant fluid by 

 means of a sterile pipette and transfer 

 to sterilized test tubes provided with 

 a sterile cotton stopper. 



(5) Slant and heat at 66 to 68°C. for 4 

 hours. 



(6) Incubate at 36° to test sterility. 



(7) After inoculation place the tubes into 

 a glass vessel half filled with distilled 

 water, and provided with a well 

 fitted glass plate. 



Sterilization: Method not given. 

 Use: Cultivation of gonococci. 

 Reference: McCann (1896 p. 1491). 



