GROUP VI. INITIALLY LIQUID MEDIA BECOMING PERMA- 

 NENTLY SOLID. SOLIDIFYING AGENT INORGANIC 



A,. Additional materials, if any, inorganic. Omelianski and Makrinoff's Soil 



Bi. Basal media; employed with the addi- Infusion Gypsum Block (Lohnis). 24So 



tion of other nutrients. -r^ ,, , t> i 



Beiierinck and van Delden's Basal 2467. Beijerinck and van Delden s Basal 



Silicate Jelly 2467 Silicate Jelly 



Smith's Basal Silicate Jelly 2468 Constituents : 



Stevens and Temple's Basal Sili- ^ ^^j^^^^ ^l^^g 



cate Jelly 2469 ^ hCI 



Doryland's Basal Silicate Jelly. . . . 2470 p^^p^^^^.^^. 



B2. Complete media. _ (1) Titrate a water glass solution, diluted 



C:. Nitrogen supplied as ammonium salts. ^.^^ ^ ^^^^^ ^^^^^^ ^^ ^^^^^^ ^.^^ 



Sleskin's Ammonium Sulphate bill- ^^^^^^ ^^.^ j^-j^^^ ^^^ ^^^^^ gl^^g 



cate Jelly 2471 ^^ ^^^^ ^^^^^ ^.^^ ^^ contraction when 



Omelianski's Ammonium Sulphate coagulation takes place. There must 



Silicate Jelly ^. .■■ ■ ■ ^4/- ^^ complete neutralization for coagu- 



Beijerinck's Ammonium Sodium ^^^.^^ .^ ^^^^^^^^ ^^ ^^ ^U^^li^^ 



Phosphate Silicate Jelly 2473 reaction 



C. Nitrogen supplied as nitrates or nitrites. ^^^ example, add 5.0 cc. of commer- 



A,. At least one constituent organic ^.^^ ^^^^^ j^^^ ^^ ^ ^1^^^ Weaker. 



B:. All additional materials of known ^.^^^^ ^.^^ 25.0 cc. of water. 



chemical composition ^^ another beaker add 10.0 cc. of N 



Doryland's Basal Glucose Silicate ^^.^ (determined for this case). 



J^lly ■■■;, ;■ o-V- ■ ■; ■ (4) mix the acid with the diluted water 



Sullivan's Basal Glycerol Silicate ^^^^ 



Je^ly • ■ ••;•;■•: - ••••■•; ■„ • ^^^^ (5) Pour'in plates. 



Fermi's Solution Silicate Jelly ^^^ p^^^ ^^^ solidified plate of NaCl by 



(Smith) ....^ .■^■■- ^47b placing in a stream of tap water. 



Doryland's Basal Acetic Acid bill- ^^^ ^^^^ ^^ ^^^^ j^^.j^^ ^^^^^ ^^^ p^^^ 



cate Jelly ;,■••■•,•••;:••,■;•■■ over the nutrient salt solution.. 



Waksman and Carey s Cellulose ^^^^ ^^^ ^^^^^^ ^^^^ ^^^ ^^^^^^ ^^ ^^^ p^^^^ 



bihca Jelly.... ^......■- ■_ • ■ ■ .^ ^ ^ excess water is evap- 



B. At least one additional constituent of - ^^^ ^^^^^ ^ ^^^ 

 unknown chemical composition. . 



C. Solidified with silicic acid. glistening upper surface 



Kuhne's Beef Extract Silicate Jelly Sterilization : Flame off the surface whereby 



(Kerry) 2479 a partial but sufficient sterilization is 



C2 Solidified by materials other than obtained. 



silicic acid (gypsum). Use: Isolation of B. oHgocarbophilus. The 



Omelianski's Gypsum Block 2480 authors reported that the medium would 



Omelianski's Nitrite Gypsum Block also support the growth of nitrifymg 



(Smith) 2481 organisms. If chalk, MgCOa or 



Omelianski's" ' ' Mannitol Gypsum NH4AIgP04 be mixed with the medium a 



Block (Percival) 2482 snow white plate will be obtained. Such 



Makrinoff's Leaf Infusion Gypsum a plate will support the growth of the 



gjQpjj. 2483 lower algae and earth diatomes. 



Makrinoff's' Soil' Infusion Gypsum Added nutrients: The authors added any 



g^Qglj 2484 desired nutrient salt solution. 



803 



