804 CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Reference: Beijerinck and van Delden anri ^.;<.k ^ . • . 



(1903 p 38) and with constant stirring 100.0 cc. 



of the sodium silicate solution (the 



2468. Smith's Basal Silicate Jelly ^^^^^^ ^^^^ being used to prevent 



Constituents : ,,, coagulation during sterilization). 



1 o ,. ... , (5) Tube. 



1. teodium silicate. /«>, t'V,^ ;^n u ii, 



2 HCl ^*ie jelly should be perfectly clear. 



Preparation: ^^^ ^LTn^ *^^ '*^"^^ ^'^"^ *° '^^^^^^ 



(1) Add drop by drop 100.0 cc. of sodium solution 'of slh Tu'" '^°^^^*^^*f ^ 



silicate (sp. gr. 1.09) to 100.0 cc. of sired but in fv '' '""'' ^' ^" 



HCl fsn frr 1 in° R«o,.,v. ^ .• • ^ ^^^^y ^^s^ containing 



fr,s r)i • 11 ,• ^^^'^ present. 



,!lH^'l,^ 7 T ?'''"'''"'' sterilization: sterilize (5) at 120- for 



until all traces of salt have been 15 minutes in the autoclave 



(3) TdTTo thrTr"- "''■■'""''' ^>-"""='- ™<iium. In pre- 



''' p.1 'ts'turta-nr sX ^ xr:iiors.tr :6 



desired composition. la+;r,,r if i ^ V . "^^°^® coagu- 



(4) Distribute in test tubes, Petri dishes Ll^tely^trL^ngtrslltt" ^^- 

 Sterilization: Sterilize by heating for ^"^f ^ ""Orients: The authors added a 



3 hours in the blood serum oven at'90°C Trl^lZl'TT^ "'"*"" °' ^^^ ^^■ 



on each of 3 successive days, or by heating p l"^ carbohydrate. 



in the autoclave at 110°C. for 15 minutes. ^®*®^^^'=«-' Stevens and Temple (1908 



Use: Synthetic solid substrata. ^' -*" 



Added nutrients: The author added any -.-^ T^ , . 



desired sterile concentrated synthetic Doryland's Basal Silicate Jelly 



culture solution. Constituents : 



Reference: Smith (1905 p. 37). 1. Water 



2469. Stevens and Temple's Basal Silicate I f^^i^ 10000 ^c. 



Jelly •^- -^Ig^O^ 0.5 g. 



•' ^ 4. CaCOaorCaO 01^ 



Constituents: r: rppW^o ^ ^ ^' 



1 „. , o. (te)-2{bOi)3 0.01 g. 



1- ^^^ter. 6. MnS04 n 01 f 



2. Sodium silicate. 7. KoSiOj ' ' »• 



3- H^^- 8. Na.SiOa 



Preparation: Preparation: 



(1) Ascertain the amount of silicic anhy. (1) Prepare a mixture of K^SiOa and 

 dride in a sample of sodium silicate, Na.SiO, dissolved in water ^o that the 

 by decomposing the silicate with con- concentration be 34.2732 g per 1 ter 

 centrated HCl, precipitating the (2) Dissolve 3, 4, 5 and 6 in HCl 



sihcic acid evaporating to dryness, (3) Standardize (2) against the silicate 



washing until the wash water shows solution so that 10 cc. o acid nei 



weifh th 'r heat to redness and tralizes 1.0 cc. of silicate jelly 



r9^ n w .1? -^ anhydride. (4) Draw (1) into a plugged burette and 



(2) Dilute the silicate so that the solu- allow to stand for Several hours'o 

 tion contains 4.0 to 5.0% silicic sterilize. 



/ON Tj l'^ , , . (^^ Draw the standardized acids into a 



stXtlso'tha^l o"' "'f f ^"f ' P^"^^^^ ^^-"^ -d allow to stnd 



strengthsothatl.Occ.neutralizeslcc. several hours to sterilize 



of he sodium silicate solution, using (6) Add 5.0 cc. of (5) to a sterile petri 



(4) To m ;c7:fr' "-f 'hT- , , '^^^ '^' *^^^ ^ « -• °f (^ -ta'ting 



W 10 104.0 cc. of this acid, add slowly rapidly to mix thoroly. 



