GROUP VII. MEDIA INITIALLY SOLID 



Ai. Solid substratum organic 



Subgroup VII-A (Med. 2486-2516) 

 As. Solid substratum inorganic 



Subgroup VII-B (Med. 2517-2543) 



SUBGROUP VII-A 



Initially Solid Organic Substrata 



Basal or Complete Media Containing 



Initially Solid Substrata of Plant or 



Animal Origin 



Ai. Solid substratum of plant origin. 

 Bi. The choice of a variety of plant tissues 

 or derivatives given. 

 Thoinot and Masselin's Vegetable 



Medium 2486 



Smith's Vegetable Medium 2487 



Reed and Cooley's Plant Material 



Medium 2488 



Smith's Plant Material Medium 2489 



Harrison's Vegetable Medium 2490 



B2. The use of a particular plant tissue or 



derivative specified. 

 Ci. Tubers or roots used. 

 Di. Potatoes employed. 

 El. No additional organic materials added. 



Simmonds' Shellaced Potato 2491 



Krannhals' Sliced Potato 2492 



Wurtz's Potato Medium 2493 



Wurtz's Potato Slants 2494 



Smith's Mashed Potato Medium. . . 2495 

 E2. Containing added organic materials. 



Wurtz's Glycerol Potato 2496 



Dalimier and Lancereaux' Opsine 



Bouillon Potato Medium 2497 



Thoinot and Masselin's Malt Potato 



Slant 2498 



Peklo's Malt Extract Potato Me- 

 dium 2499 



Harras' Tissue Potato Medium 2500 



D2. Other tubers or roots employed. 



Matzuschita's Carrot Medium 2501 



Smith's Beet Medium 2502 



Smith's Onion Medium 2503 



Roger's Artichoke Medium 2504 



C2. Fruits used. 



Smith's Cocoanut Medium 2505 



Smith's Orange Medium 2506 



Duval's Basal Banana Medium 2507 



C3. Bread, macaroni, noodles, etc. used. 

 Heinemann's Bread Paste Medium. 2508 



Logerheim's Macaroni Medium 2509 



Logerheim's Noodle Medium 2510 



C4. Filter paper used. 



Lohnis Filter Paper Medium 2511 



Cunningham's Nitrate Filter Paper 



Medium 2512 



Malenkovic's Nitrate Sawdust Me- 



dium 



2513 



A 2. Solid substratum of animal origin. ^ 

 Bi. Animal tissue without other materials. 



Besson's Animal Organ Medium. . . . 2514 

 B2. Animal organs or tissue with other 



materials. 



Picker's Glycerol Tissue Medium. . . 2515 



Frugoni's Glycerol Bouillon Tissue 

 Medium (Abel) 2516 



2486. Thoinot and Masselin's Vegetable 

 Medium 



Constituents 

 Preparation 



1. Potato or other vegetable. 



(1) Wash good sound potatoes thoroly. 



(2) Soak for an hour in a 1 to 1000 solution 

 of sublimate. 



(3) Wash a large crystallizing dish with 

 a 1 to 1000 sublimate solution. 



(4) Place a filter paper moistened with 

 the sublimate solution in the bottom 

 of the dish. 



(5) Cut the potato into two equal halves, 

 using a sterile knife. 



(6) Place each half in the crystallizing 

 dish prepared as indicated above. 

 Put the cover in place. 



(7) The potatoes may be cut into discs 

 and placed in Petri dishes. 



Sterilization: Autoclave at 100°C. for one 

 hour and then at 115° for 15 minutes 

 at least. 



Use: General culture medium. 



813 



