814 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Variants: The authors prepared turnips, 

 black radishes or carrots in the same 

 manner as potatoes. 



Reference: Thoinot and Masselin (1902 

 pp. 48, 81). 



2487. Smith's Vegetable Medium 



Constituents: 1. Potato or other vegetable. 

 Preparation : 



(1) Prepare slant cylinders of one of the 

 following: 

 Potato banana 



sweet potato cocoanut 



carrot peanuts 



sugar beet brazilnuts 



turnip apple 



radish pear 



salsify quince 



parsnip pineapple 



onion 

 (2) Place in test tubes and half cover 

 by the addition of distilled water. 

 The pieces of vegetable may be placed 

 in Roux tubes, or tubes containing a 

 wad of cotton. 

 Sterilization: Sterilize on each of 3 suc- 

 cessive days for 20 minutes in streaming 

 steam. 

 Use : Cultivation of plant parasites. 

 Reference: Smith (1905 p. 40). 



2488. Reed and Cooley's Plant Material 



Medium 

 Constituents : 



1. Water. 



2. Corn meal or other plant derivative. 

 Preparation : 



(1) Place corn meal in test tubes. 



(2) Add water. 



(3) Cook in the moist heat of the steri- 

 lizer. 



Sterilization: See (3) above. 



Use: Cultivation of Heterosporium va- 



r labile. 

 Variants: The authors substituted rice, 



shredded cocoanut, or spinach leaves for 



corn meal. 

 Reference: Reed and Cooley (1911-12 



p. 50). 



2489. Smith's Plant Material Medium 

 Constituents : 



1. Radish, 

 horse radish. 



yellow turnip, 

 white turnip, 

 cabbage leaves or 

 cauliflower. 

 Preparation : 



(1) Take slices of radish, horse radish 

 roots, yellow and white turnip, or the 

 base of cabbage or cauliflower leaves, 

 and place in sterile test tubes. 



(2) Nearly cover with distilled water. 

 Sterilization: Steam on 3 successive days 



for a few minutes. 



Use: Cultivation of Pseudomonas campes- 

 tris (Pammel). Author reported that 

 growth was prompt and abundant on all 

 material except horse radish. Growth on 

 horse radish slow but finally abundant. 

 Growth yellow in color. 



Reference: Smith (1897 p. 481). 



2490. Harrison's Vegetable Medium 



Constituents: 1. Potato or other vegetable. 



Preparation : 

 (1) Select sound specimens of average 

 size of one of the following vegetables: 

 potato, Jerusalem artichoke, cucum- 

 ber, red carrot, horse radish (edible 

 portion), radish, red beet, sugar 

 beet, parsnip, cauliflower, cabbage, 

 celery, mangelwurzel, Swede turnip, 

 white turnip, kohlrabi, salsify, green 

 tomato, ripe tomato, onion. 



(2) In case of roots, wash and scrub in 

 running water, soak for an hour in 

 sublimate 1:1000. 



(3) Cut thick slices 20-40 mm. thick 

 with sterile knives and place in deep 

 Petri dishes at the bottom of which 

 is a filter paper saturated with 

 bichloride of mercury solution 

 (1-5000). 



(4) In case of celery, cabbage, cauli- 

 flower, etc., wash the whole plant 

 thoroly and dry. 



(5) Cut down the middle with a sterilized 

 knife. 



(6) Place in large double dishes. 

 Sterilization: Not specified. 



Use: Cultivation of Bacillus solanisaprus 

 (causing bacterial rot in potatoes). 

 Author reported that generally the bacil- 

 lus developed on the vegetables listed. 



Reference: Harrison (1907 pp. 172, 384). 



