CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



817 



(e) Pitfield gave the following method of 

 preparation: 



(1) Cut semi-cylinders from well 

 washed large potatoes using a 

 Ravenel potato cutter. 



(2) Wash the potato semi-cylinders in 

 a 1:10,000 bichloride of mercury 

 solution and then in running water 

 over night. 



(3) Thrust cotton in the bottom of a 

 tube and wet with distilled water. 



(4) Place the potato cylinders on the 

 cotton. 



(5) Plug._ 



(6) Sterilize in the autoclave (time or 

 temperature not specified). 



(f) Other investigators prepared potato 

 slants in essentially the same manner. 

 Their methods were not sufficiently 

 different to warrant an individual 

 discussion. 



References: Wurtz (1897 p. 32), Thoinot 

 and Masselin (1902 p. 50), Smith (1902 

 p. 75), Frost (1903 p. 18), Heinemann 

 (1905 p. 27), Roux and Rochaix (1911 

 p. 120), Abel (1912 p. 28), Lohnis (1913 

 p. 20), Roddy (1917 p. 46), Ball (1919 

 pp. 72, 73), Percival (1920 p. 58), Bezangon 

 (1920 p. 114), Besson (1920 p. 56), Roux 

 and Rochaix (1921 p. 121), Dopter and 

 Sacquepee (1921 p. 128), Giltner (1921 

 p. 26), Abbott (1921 p. 132), Harvey 

 (1921-22 p. 118), Pitfield (1922 p. 117), 

 Stitt (1923 p. 41), Klimmer (1923 p. 205), 

 Park, Williams and Krumwiede (1924 

 p. 121), Cunningham (1924 p. 17). 



2495. Smith's Mashed Potato Medium 



Constituents : 



1. Potato. 

 Preparation : 



(1) Mash thoroly cleaned and boiled 

 potatoes. 



(2) Spread in Petri dishes or tube. 

 Sterilization: Method not given. 



Use: Owen cultivated B. saccharalis on 



mashed potatoes. 

 Variants : 



(a) Abel crushed boiled potatoes to a 

 pulp adding a little water or milk, 

 and sterilized in a steamer. 



(b) Ball prepared a similar medium as 

 follows: 



(1) Mash peeled potatoes with distilled 

 water until thick. 



(2) Sterilize in flasks for 45 minutes on 

 3 successive days. 



(c) Tanner gave the following method of 

 preparation: 



(1) Clean large potatoes and cut into 

 slices. 



(2) Boil until soft. 



(3) Grate, or press thru ricer. 



(4) Distribute in layers in Petri dishes. 



(5) Sterilize at 120° for 20 minutes. 



(6) The slices may be placed in the 

 Petri dishes without mashing if 

 desired. 



(d) Bushnell used the following medium 

 to study chemical changes produced 

 by the B. sporogenes group: 



(1) Peel potato and pass thru meat 

 grinder. Wash ground potato in 

 running water several hours. 



(2) Sterilize at 20 pounds for 30 minutes. 



(3) No adjustment of reaction given. 

 References: Smith (1902 p. 76), Owen (1916 



p. 243), Abel (1912 p. 28), Ball (1919 p. 73), 

 Tanner (1919 p. 60), Besson (1920 p. 57), 

 Dopter and Sacquepee (1921 p. 129), 

 Abbott (1921 p. 132), Harvey (1921-22 

 p. 119), Bushnell (1922 p. 381). 



2496. Wurtz's Glycerol Potato 



Constituents : 



1. Potato. 



2. Glycerol (8.0%). 



Preparation: (1) Add sufficient sterile 8.0% 

 glycerol to a Roux tube containing a 

 piece of potato, so that the bottom of 

 the potato slant is immersed in the 

 glycerol. 



Sterilization: Method not given. 



Use : Cultivation of tubercle bacilli. Peklo 

 cultivated plant actinomyces on a similar 

 medium. 



Variants : The following investigators have 

 prepared glycerol potato as indicated, 

 (a) Matzuschita (1899). 



(1) Cut pieces of potato with a cylin- 

 drical borer. 



(2) Cut away the end portion. 



(3) Halve the cylindrical pieces of 

 potato and place in plates, con- 

 taining glycerol (or water). The 

 pieces may have been soaked in 

 1.0% soda solution for 15 minutes. 



