CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



819 



(2) Place each piece of potato in a 

 Roux tube. 



(3) Sterilize. 



(i) Roux and Rochaix (1911). 



(1) Add sufficient quantity of sterile 

 4.0% glycerol solution to sterile 

 potato slants so that the lower 

 half of the potato is immersed in 

 the glycerol solution, 

 (j) Anzilotti (Kolle and Wasserman) 

 (1912). 



(1) Boil potato slants in a 6.0% solu- 

 tion of glycerol made alkaline by 

 the addition of NaoCOs. The 

 boiling is continued until the pota- 

 toes have swollen (usually about 

 20 minutes). If the reaction 

 changes, add more NaaCOs. 



(2) Place the slants so prepared in 

 Roux tubes, containing a little 

 6.0% glycerol bouillon. 



(3) Sterilize at 120°C. for 20 minutes 

 (k) Harvey (1921-22). 



(1) Cover a half cylinder of potato 

 with 6.0% glycerol. 



(2) Steam 30 minutes. 



(3) Discard the fluid covering the 

 potato. 



(4) Sterilize 30 minutes at 100°C. 

 (1) Harvey (1921-22). 



(1) Scrub a number of potatoes of un- 

 broken skin thoroly with a stiff 

 brush. 



(2) Cut off the outer skin. 



(3) Cut out of the potato with a potato 

 borer a cylinder of about 3 to 

 4 inches in diameter. 



(4) Divide the cylinder diagonally 

 into two parts. 



(5) Wash in running water over night. 



(6) Soak the cut out cylinder in 6.0% 

 glycerol and soak the wool in the 

 test tube also in 6.0% glj'cerol. 



(7) Sterilize 20 minutes at 120°C. 

 (m) Harvey (1921-22). 



(1) Soak diagonally cut cylinders of 

 potato in 1-1000 sodium carbonate 

 24 hours. 



(2) Transfer to 5.0% glycerol 24 hours. 



(3) Distribute in test tubes containing 

 a pledget of wool at the bottom. 



(4) Fill up the tubes with the 5.0% 

 glycerol. 



(5) Sterilize 3 days at 100°C. 



(6) Pour off the glycerol at the time 

 of use. 

 (n) Dopter and Sacquepee (1921). 



(1) Scrub large white whole potatoes 

 in water. 



(2) Cut cylindrical pieces from the 

 potato using a cork borer. 



(3) Allow the potatoes to soak in a 

 6 to 15 part per 100 glycerol solu- 

 tion for 48 hours. 



(4) Add glycerol solution to Roux 

 tubes until the solution is level 

 with the constriction. 



(5) Place a cylinder of potato in each, 

 tube. 



(6) Sterilize (method not given), 

 (o) Klimmer (1923). 



(1) Thoroly wash and scrub medium 

 sized, sound unimpaired potatoes 

 with a scrubbing brush, soap and 

 water. 



(2) Carefully remove all eyes and 

 scabs. 



(3) Soak the potatoes for 1 to 2 hours 

 in a 1.0% sublimate solution. 



(4) Make the potato slants slightly 

 alkaline using soda solution. 



(5) Place each piece of potato in a 

 Roux tube. 



(6) Add 5.0 to 6.0% glycerol solution 

 to each tube until half the potato 

 is immersed in the liquid. 



(7) Cook for about 20 minutes in the 

 steamer until the potato is soft. 



(8) Sterilize for 45 minutes in the 

 steamer. 



(p) Cunningham (1924). 



(1) Place a small piece of cotton-wool 

 in the bottom of each of a number 

 of dry test tubes. 



(2) Moisten each plug with 2 or 3 

 drops of water (not more). 



(3) Scrub several sound potatoes 

 under the tap and peel carefully. 



(4) Cut into pieces about 1x1x4 

 to 5 cm. 



(5) Divide each piece into large wedge- 

 shaped portions by cutting the 

 pieces diagonally. 



(6) Drop each piece as it is cut into a 

 beaker of clean water. 



(7) Cover with 6.0% glycerol in dis- 

 tilled water in a beaker. 



(8) Steam for 30 minutes. 



