820 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(9) Pour off the glycerol and place 

 one of the wedges in each of the 

 prepared tubes. The broad end 

 of the wedge at the bottom. 

 (10) Sterilize in the autoclave at 

 22.5 pounds pressure, 

 (q) Park, Williams and Krumwiede 

 (1924). 



(1) Scrub large white potato under 

 running water. 



(2) Cut cylinders by means of an 

 apple corer. 



(3) Cut the skin off the ends of the 

 potato cylinders. 



(4) Make 2 wedge shapes from each 

 cylinder by cutting thru obliquely. 



(5) Soak the potatoes over night in a 

 1:1000 Na2C03 solution. 



(6) Drain. 



(7) Cover with a 5.0% glycerol solu- 

 tion for 24 hours. 



(8) Place the pieces in test tubes one 

 inch or more in diameter. 



(9) Add a little of the glycerol solu- 

 tion, or water to each tube. 



(10) Sterilize in the autoclave at 

 15 pounds pressure for 30 minutes. 

 References: Wurtz (1897 p. 46), Matzu- 

 schita (1899 p. 128), Thoinot and Masselin 

 (1902 p. 53)', Smith (1902 p. 78), Heine- 

 mann (1905 p. 129), Anzilotti (1906 p. 766), 

 Peklo (1910 p. 473), Park and Krumwiede 

 (1910 p. 215), Roux and Rochaix (1911 

 p. 122), Kolle and Wasserman (1912 

 p. 408), Harvey (1921-22 p. 118), Dopter 

 and Sacqu6p6e (1921 p. 129), Klimmer 

 (1923 p. 206), Cunningham (1924 p. 166), 

 Park, Williams and Krumwiede (1924 

 p. 122). 



2497. Dalimier and Lancereaux' Opsine 

 Bouillon Potato Medium 



Constituents : 



1. Bouillon. 



2. Opsine. 



3. Potato. 



4. Glycerol. 

 Preparation : 



(1) Prepare bouillon with a slightly alka- 

 line reaction. 



(2) Add opsine (a commercial mixture of 

 amino acid) and glycerol (amount 

 not given to (1). 



(3) Place potato slants in Roux tubes. 



(4) Pour (2) over (3). 

 Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli and 

 other saprophytic and parasitic forms. 



Reference: Dalimier and Lancereaux (1913 

 p. 419). 



2498. Thoinot and Masselin's Malt 



Potato Slant 



Constituents : 



1. Water 100.0 cc. 



2. Malt dust 10.0 g. 



3. Sucrose 5.0 g. 



4. Glycerol 2.0 g. 



5. Potato 

 Preparation : 



(1) Prepare an infusion from 10 parts 

 malt dust and 100 parts water. 



(2) Add 5 parts sucrose and 2 parts 

 glycerol to (1). 



(3) Prepare potato slants in Roux tubes. 



(4) Add (2) to each tube of sterile (3) 

 to fill the tube to the constriction in 

 the tube. 



(5) Slant the tube after inoculation so 

 as to have the potato in contact with 

 the liquid. 



Sterilization: Method of sterilization of 



potato slant not given. Sterilization of 



(2) not specified. 

 Use: Cultivation of streptothrix, tubercle 



bacilli and molds. 

 Reference: Thoinot and Masselin (1902 



p. 52). 



2499. Peklo's Malt Extract Medium 



Constituents : 



1. Distilled water 



2. Malt 



3. K2HPO4 8.0 g. 



4. K2CO3 6.0 g. 



5. Potato 

 Preparation : 



(1) Thoroly mix malt and distilled water. 



(2) Boil for 1 hour and allow to stand at 

 60°C. for 24 hours. 



(3) Boil again for one hour. 



(4) Filter. 



(5) Dilute the filtrate with distilled water 

 (amount not given). 



(6) To 700.0 cc. of sterile (5) add 780.0 cc. 

 distilled water, 8.0 g. K2HPO4 and 

 6.0 g. K2CO3. 



