CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



821 



(7) Impregnate potato slants with sterile 

 (6). 



Sterilization: Sterilize (5) by heating for 

 several successive days and filter after 

 each heating. Sterilization of potato 

 slants not specified. Sterilize (7) method 

 not given. 



Use: Cultivation of plant actinomyces. 



Reference: Peklo (1910 p. 473). 



2500. Harrass' Tissue Potato Medium 

 Constituents: 



1. Potato. 



2. Liver. 

 Preparation: 



(1) Grind brain or liver in a meat chop- 

 ping machine. 



(2) Peel well brushed potatoes and cut 



into small cubes. 



(3) Cook the potato cubes well in water. 



(4) Add brain or liver pulp to the well- 

 cooked potato cubes and pound to 

 a pulp. 



(5) Distribute in Petri dishes. 

 Sterilization: Sterilize 1.5 to 2 hours in 



streaming steam. If the consistency is 

 too thin place in a drying incubator until 

 the desired consistency is reached. 



Use: Cultivation of obligate anaerobes. 



Variants: Author used brain instead of 

 liver. 



Reference: Harrass (1906 p. 2237). 



2501. Matzuschita's Carrot Medium 

 Constituent: 



1. Carrot. 

 Preparation : 



(1) Shove a cylindrical borer thru a 

 carrot. 



(2) Cut away the end portions of the 

 cylindrical piece of carrot. 



(3) Halve the cylindrical piece of carrot. 



(4) Place in plates containing water. 

 Sterilization: Sterilize on each of 3 suc- 

 cessive days for 30 minutes in the steamer. 



Use: Cultivation of tubercle bacilli, mam- 

 malian and chicken. Other investigators 

 used similar media for a variety of 

 purposes. 

 Variants : 



(a) The author specified that carrot 

 may be soaked in a 1.0% watery 

 solution of soda for | hour before 

 sterilizing. This gives an alkaline 



carrot. Growth generally more lu.xu- 

 riant on alkaline medium. 



(b) The author used glycerol in the plates 

 containing the carrot instead of 

 water as indicated in step (4) above. 



(c) Roux and Rochai.v prepared carrot in 

 exactly the same manner as potato. 

 See variant (h) and (i), medium 2496 

 and medium 91. 



(d) Dopter and Sacquepee gave the fol- 

 lowing method of preparation. 



(1) Scrub large whole carrots with 

 water. 



(2) Cut cylindrical pieces. 



(3) Wash the cylinders thoroly with 

 water. 



(4) Dry on filter paper. 



(5) Place in Roux tubes. 



(6) Sterilize at 120° for 20 minutes. 



(7) The carrots may be cut in discs, 

 placed in Petri dishes and sterilized. 



(e) Davis used sterilized sliced carrots to 

 demonstrate chromogenesis. He re- 

 ported that a deep shiny black pig- 

 ment was formed that did not diffuse 

 into the carrot. 



References: Matzuschita (1899 p. 128), 

 Roux and Rochaix (1911 p. 123), Dopter 

 and Sacquepee (1911 p. 129), Davis (1915 

 p. 179). 



2502. Smith's Beet Medium 



Constituents: 



1. Beet. 

 Preparation : 



(1) Pare and cut small red turnip-beets 

 into long slices and place in sterile 

 test tubes. 



(2) Nearly cover with distilled water. 

 Sterilization: Steam on 3 successive days 



for 20 to 30 minutes. 



Use: Cultivation of Pseudomonas campes- 

 tris (Pammel). Author reported that on 

 the surface in the air organism made a 

 prompt and copious pale yellow growth, 

 while the fluid was changed to its original 

 bright yellow color. Matzuschita culti- 

 vated mammalian and chicken tubercle 

 bacilli on a similar medium. 



Variants: Matzuschita prepared the me- 

 dium as follows: 



(1) Shove a cylindrical borer thru a beet. 



(2) Cut away the end portions of the 

 cylindrical piece of beet. 



