822 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(3) Halve the cylindrical piece of beet. 



(4) Place in plates containing glycerol 

 (or water). (The pieces may have 

 been previously soaked in 1.0% soda 

 solution for 15 minutes.) 



(5) Sterilize in the steamer on each of 

 3 successive days for 30 minutes. 



References: Smith (1897 p. 481), Matzu- 

 schita (1899 p. 129). 



2503. Smith's Onion Medium 



Constituents : 



1. Onion. 

 Preparation : 



(1) Slice a sweet Bermuda onion and 

 place in test tubes. 



(2) Nearly cover the onion with dis- 

 tilled water. 



Sterilization: Steam for a few minutes on 

 3 successive days. 



Use: Cultivation of Pseudomonas campes- 

 tris (Pammel). Author reported that 

 organism formed a pale yellow slime and 

 a rather copious pale precipitate. 



Reference: Smith (1897 p. 482). 



2504. Roger's Artichoke Medium 



Constituents : 



1. Artichoke. 

 Preparation : 



(1) Wash the leaves of artichoke, and 

 cut the bottom into small pieces. 



(2) Place the small pieces, the leaves on 

 top into tubes, whose ends are plugged 

 with moist cotton. 



Sterilization: Sterilize at 115° for 15 

 minutes. 



Use: Cultivation of streptococci, diph- 

 theria bacilli. Bacillus subtilis, Bacillus 

 prodigiosus and others. 



Variants : 



(a) Roux and Rochaix sterilized at 115°C. 

 for 20 minutes. 



(b) Besson added a little water to each 

 tube. 



(c) Bezangon sterilized at 175°C., time 

 not given. 



References: Roger (1898 p. 769), Roux and 

 Rochaix (1911 p. 123), Besson (1920 p. 51), 

 Bezangon (1920 p. 115), Dopter and 

 Sacqu6p6e (1921 p. 129). 



2505. Smith's Coconut Medium 



Constituents : 

 1. Coconut. 



Preparation : 



(1) Cut the flesh of a coconut into strips. 



(2) Place the strips into sterile test tubes. 

 Sterilization: Steam on 3 successive days 



for a few minutes. 



Use: Cultivation of Pseudomonas campes- 

 tris (Pammel). Author reported that 

 the organism produced a pale yellow 

 growth on the surface. Schouten culti- 

 vated fungi and yeast on coconut. 



References: Smith (1897 p. 481), Schouten 

 (1916 p. 480). 



2506. Smith's Orange Medium 



Constituents : 

 1. Orange. 

 Preparation : 



(1) Segment a rather tart but palatable 

 California Navel orange. 



(2) Place into sterile cotton plugged test 

 tubes and cover with distilled water. 



Sterilization: Steam on 3 successive days 



for several minutes. 

 Use: Cultivation of Pseudomonas cam- 



pestris (Pammel). Author reported no 



growth. 

 Reference: Smith (1897 p. 480). 



2507. Duval's Basal Banana Medium 



Constituents : 

 1. Banana. 

 Preparation : 



(1) Carefully remove the rind from the 

 fruit portion of fully matured green 

 bananas, using every caution to avoid 

 contamination. 



(2) Slant one surface of the fruit with a 

 sterile sharp knife and place into 

 sterile glass cylinders containing cot- 

 ton plugs saturated with sterile 

 distilled water. 



(3) Prepare 1.0% solutions of one of the 

 added nutrients. 



(4) Add one of sterile (3) to the surface 

 of the banana. 



Sterilization: Sterilize (3) by passing thru 

 a Berkefeld. 



Use: Cultivation of B. leprae. Author re- 

 ported that cystein gave excellent results. 



Added nutrients: The author used a 1.0% 

 solution of tryptophane, cystein (pre- 

 pared from protein) or leucine. 



Variants: The author used the added nu- 

 trients in combination. 



Reference: Duval (1910 p. 655). 



