CULTURE MEDIA FOR CULTIVATIOX OF MICROORGANISMS 823 



2508. Heinemann's Bread Paste Medium (5) Pour off all the water and plug the 



Constituents: tubes. 



1. Water. Sterilization: Sterilize in the usual way in 



2. Bread. the steamer. 



Preparation : Use : Cultivation of chromogenic and other 



(1) Cut bread into slices. bacteria. Author reported the medium 



(2) Dry in the oven. as being a good substitute for potato. 



(3) Pulverize. May be used in Petri dishes instead of 



(4) Distribute in 100.0 cc. flasks until the *"^^^- 



layer on the bottom is half an inch Reference: Logerheim (1892 p. 147). 



thick. 



/rNp.^„.' ,, , . ^ , 2510. Logerheim's Noodle Medium 



(5j (gradually run water in to cover the -icuiuiu 



surface of the bread. Treat noodles in exactly the same manner 



Sterilization: Sterilize in the Arnold. ^^ macaroni in preceding medium 2509. 



Use: Cultivation of molds and bacteria. ocr,, inh^ic. Tr-i+ n ti/t j. 



Variants: Lohnis Filter Paper Medium 



(a) Tanner soaked slices of bread in dis- Constituents : 

 tilled water and sterilized at 115°C. ^- ^^^'ter paper. 



(b) Tanner added 2.5 parts of water to 1 2. MgNH4P04. 



part by weight of ground bread ^- K2HPO4 (0.05% soln.). 



crumbs and sterilized the mixture at Preparation: 



115°C. for 20 minutes. (1) Place two filter papers in Petri dishes. 



(c) Ball sterilized a paste prepared from ^^^ Sprinkle MgNH4P04 between the 

 bread crumbs and water in the filter papers and moisten with a 0.05% 

 steamer on each of 3 successive days aqueous solution of K2HPO4. 



for 45 minutes. Sterilization : Not specified. 



(d) Lloyd, Clark and McCrea cultivated ^^®* Cultivation of cellulose decomposers 

 Flugge's Mesentericus group on a me- ^'"^^ *1^^ ^<^il- 



diurn prepared as follows: References: Lohnis (1913 p. 104), Cunning- 



(1) Cut small pieces of bread a quarter ^^^ (^924 p. 129). 



of an inch thick and sterilize them 2512. Cunningham's Nitrate Filter Paoer 



in Petri dishes in the autoclave for -J , ""^^^^ ^^^^"^ ^^P®*^ 



20 minutes at 115°C. ^^^^""^ 



References: Heinemann (1905 p. 128), Constituents: 



Abel (1912 p. 29), Lohnis (1913 p. 88)^ 1- Water (tap) 1000.0 cc. 



Tanner (1919 p. 59), Ball (1919 p. 73) 2. K2HPO4 0.5 g. 



Besson (1920 p. 58), Lloyd and McCrea 3. NH4NO3 0.5 g. 



(1920-21 p. 383), Harvey (1921-22 p. 120), ^- Cellulose 



Klimmer (1923 p. 207). ' Preparation: 



(1) Place two discs of filter paper in a 



2509. Logerheim's Macaroni Medium P^^^i dish and add a little rich 



garden soil. 



Constituents : (2) j^^.^^^^^ ^ and 3 in 1. 



1. Macaroni. (3) Moisten (1) and keep moist by the 



Preparation: addition of (2). 



(1) Secure white macaroni about 5.0 mm. Sterilization: Not specified 



in thickness with a 3.0 mm. caliber. Use : Decomposition of cellulose by fungi. 



(2) Break m pieces about 4.5 cm. long Reference: Cunningham (1924 p 141) 

 and place in sterile test tubes. 



(3) Fill the tubes with water so that the ^^^^- Malenkovic's Nitrate Sawdust 

 macaroni is immersed and about Medium 



1.0 cm. of water above the macaroni. Constituents: 



(4) Boil until the macaroni swells (re- 1. Water 1000 cc 



quires about 15 minutes). 2. KNO3 I5 



