824 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. K2HPO4 1.5 g. 



4. NH4H2PO4 1.0 g. 



5. MgS04... 0.5 g. 



6. Sawdust 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Exhaust sawdust with water, cold 

 ammonia or boiling KOH (method 

 not given). (When exhausting with 

 KOH have the wood free from xylan.) 



(3) Moisten (2) with (1). 

 Sterilization: Not specified. 



Use: Cultivation of Coniphpora cerebella 

 {Corticium putaneum). Author reported 

 that growth was generally good. 



Variants: The author prepared cellulose 

 from beechwood according to Lange's 

 method (by heating with potassium ace- 

 tate, details of method not given), or 

 prepared lignin acid by Lange's method, 

 and substituted either cellulose or lignin 

 acid for sawdust. 



Reference: Malenkovic (1906 p. 412). 



2514. Besson's Animal Organ Medium 



Constituents : 



1. Animal organs. 

 Preparation : 



(1) Obtain the organs (placenta, liver, 

 spleen, kidney, etc.) under as nearly 

 aseptic conditions as possible and 

 place in a sterile container. 



(2) Sear the surface of the organs with 

 a hot metal. 



(3) Cut small pieces from the organ, using 

 a sterilized knife and pincher. 



(4) Distribute in sterile Petri dishes or 

 large sterile test tubes. 



(5) Incubate for 2 days at 37°C. to test 

 sterility. 



Sterilization: The whole method of prepa- 

 ration should be carried out under as 

 nearly aseptic conditions as possible. 

 Use : General culture medium. 

 Variants : 



(a) Harvey cultivated spirochetes and 

 anaerobic organisms on organs pre- 

 pared as follows: 



(1) Kill a rabbit. 



(2) Fasten it on a post-mortem board 

 on its back with limbs fully ex- 

 tended and pinned to the board. 



(3) Wet the hair over thorax and ab- 

 domen with 2.0% lysol or other 

 antiseptic. 



(4) Cut the hair as short as possible 

 and again wet the body surface 

 with antiseptic solution. 



(5) Make a mesial incision with sterile 

 instruments thru the skin, to ex- 

 tend over thorax and abdomen. 



(6) Dissect off the skin in flaps to ex- 

 pose the anterior surface of thorax 

 and abdomen completely. 



(7) Use a fresh set of sterile instru- 

 ments and open the abdomen. 



(8) Remove with sterile precautions 

 the organs, liver, kidneys, spleen 

 and testicles. 



Note: Organs and tissues ob- 

 tained at ordinary post mortem 

 examination may also be used 

 after sterilization. 



(9) Place in sterile Petri dishes. 



(10) Open the thorax with a fresh set 

 of sterile instruments. 



Note: If the thorax as well as 

 the abdomen is to be opened, 

 the former should be opened first. 



(11) Remove the lungs and place in 

 sterile Petri dishes. 



(12) Cut up the organs obtained with 

 sterile precautions. 



(b) Harvey removed the lungs, liver, 

 kidney, spleen and testicles from a 

 rabbit prepared as indicated above. 

 Treat the organs as follows: 



(1) Sear the surface of the organs. 



(2) Cut out suitably sized portions 

 with sterile forceps and scissors. 



(3) Place in sterile test tubes or Petri 

 dishes. 



References: Besson (1920 p. 55), Harvey 

 (1921-22 p. 97). 



2515. Picker's Glycerol Tissue Medium 



Constituents : 



1. Glycerol 3.0% solution. 



2. Brain. 

 Preparation : 



(1) Place brain tissue in an empty con- 

 tainer and steam for 60 to 90 minutes. 



(2) Remove from steamer and slice into 

 pieces. 



(3) Place the pieces in plates or tubes. 



(4) Add to the tube about 10 drops of a 

 3.0% glycerol and to the plate 15 to 

 20 drops. 



Sterilization : Sterilize twice for 30 minutes 

 each time in steam. 



