CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



825 



Use: Cultivation of tubercle bacilli, 

 Author reported that human, sheep, beef, 

 calf and horse brain were used but all 

 gave same results. 



Variants : 



(a) Frugoni prepared a similar medium 

 as follows : 



(1) Place animal tissue (lung of rabbit 



or dog) in the autoclave for 

 45 minutes. 



(2) Cut in small prism-like pieces under 



aseptic conditions from the sterile 

 tissue. 



(3) Allow the pieces to soak in 6-8.0% 

 glycerol solution. 



(4) Place the glycerolated tissue in 

 sterile Roux tubes. 



(5) Add 6-8.0% glycerol solution to the 

 tube so that the tissue is touching 

 the solution. 



(b) Harvey gave the following method of 

 preparation: 



(1) Treat cut up portions of organs 

 sterilely removed from a rabbit 

 specially killed for the purpose 

 (see variant (a) medium 2514) with 

 6.0% glycerolated 0.85% sterile salt 

 solution for one hour. 



(2) Prepare sterile test tubes contain- 

 ing a 2 cm. length of glass tubing as 

 support for the tissue. 



(3) Add 6.0% glycerolated 0.85 sterile 

 salt solution to the test tubes to the 

 top of the supporting glass rod. 



(4) Transfer a suitably sized portion of 

 glycerolated organ tissue to each 

 test tube to rest on the glass tubing 

 support. 



(5) Sterilize 30 minutes at 120°C. 

 References: Ficker (1900 p. 593), Frugoni 



(1910 p. 557), Kolle and Wassermann (1912 

 p. 409), Harvey (1921-22 p. 98). 



2516. Frugoni's Glycerol Bouillon Tissue 

 Medium (Ficker) 

 Constituents: 



1. Tissue (rabbit). 



2. Glycerol bouillon. 

 Preparation : 



(1) Soak fresh rabbit lung or other tissue 

 for one hour in 0.85% NaCl solution 

 containing 6.0% glycerol. 



(2) Support sterile (1) over the surface 

 of 6.0% glycerol bouillon so that the 



tissue is kept moist by capillary at- 

 traction and condensation. (Post 

 mortem tissue may be used after 

 sterilizing by boiling or in the auto- 

 clave.) 



Sterilization: Sterilize in the autoclave at 

 120 °C. for 30 to 45 minutes. 



Use: Cultivation of tubercle bacilli. 



Variants: Klimmer prepared the medium 

 as follows: 



(1) Heat the liver and lungs of a dog or 

 rabbit in the autoclave for 30 to 45 

 minutes. 



(2) Cut (1) into strips or prisms. 



(3) Soak (2) in 6 to 8.0% glycerol solution 

 for 1 to 2 hours. 



(4) Place in containers so that the tissue 

 is just touching 6.0 to 8.0% glycerol 

 bouillon. It may be necessary to 

 have the pieces of tissue rest on a 

 piece of glass rod. 



References: Ficker (1900 p. 593), Frugoni 

 (1910 p. 557), Abel (1912 p. 98), Klimmer 

 (1923 p. 201). 



SUBGROUP VII-B 

 Initially Solid Inorganic Substrata 

 Basal or Complete Media Containing 

 Initially Solid Inorganic Materials 

 Ai. Sand only employed. 

 Bi. Organic constituents not added. 

 Ci. Nitrogen supplied as ammonium salts. 

 Munter's Basal Ammonium Nitrate 



Sand Medium 2517 



Gowda's Ammonium Sulphate Sand 



Medium 25i« 



Wichers' Ammonium Sulphate Sand 



Medium 2519 



C2. Nitrogen supplied as nitrites or ni- 

 trates. 

 Malenkovic's Basal Nitrate Sand 



Medium 2^)20 



Stigell's Nitrate Sand Medium 2521 



B2. Organic constituents added. 

 Ci. All^ additional constituents of known 

 chemical composition. 

 Krilger and Schneidewind's Basal 



Glucose Sand Medium 2522 



Ashby's Basal Mannitol Sand Me- 

 dium (Reed and Williams) 2523 



Miinter's Basal Carbohydrate As- 

 paragin Sand Medium 2524 



