826 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



C2. At least one additional constituent of 

 unknown chemical composition. 

 Heinze's Beerwort Sand Medium. . . 2525 

 Kriiger and Schneidewind's Extract 



Broth Sand Medium (Heinze) 2526 



Stigell's Bouillon Sand Medium 2527 



Heinze's Loam Sand Medium 2528 



A2. Materials other than sand present. 

 Bi. A variety of "starra" (solid) substrata 



specified. 

 B2. A single starra (solid) substratum 

 specified. 



Mortensen's "Stone" media 2529 



Ci. Organic materials not added. 



Wojtkiewicz's Soil Medium 2530 



Makrinoff's Carbonate Soil Me- 

 dium 2531 



Wichers' Ammonium Sulphate Per- 



mutite Medium. 2532 



Rossler's Iron Sulphate Brick Me- 

 dium 2533 



Mortensen's Basal Nitrate "Starre" 



Medium 2534 



C2. Organic materials added. 

 Di. All additional constituents of known 

 chemical composition. 



Greaves' Mannitol Soil Medium 2535 



Vierling's Cellulose Soil Medium. . . 2536 

 Mortensen's Ammonium Sulphate 



"Starre" Medium 2537 



D2- At least one additional constituent of 



unknown chemical composition. 

 El. Peptone added. 

 Zikes' Glucose Peptone Soil Medium 2538 

 Mortensen's Peptone "Starre" Me- 

 dium 2539 



E2. Peptone not added. 



Lipman's Dried Blood Soil Medium. 2540 

 Greaves' Dried Blood Soil Medium. 2541 

 Schonbrunn's Horn Meal Soil Me- 

 dium 2542 



Lohnis and Green's Phosphate Meal 

 Medium 2543 



2517. Miinter's Basal Ammonium Nitrate 

 Sand Medium 



Constituents : 



1. Water 1000.0 cc. 



2. MgSOi 0.5 g. 



3. NaCl 0.5 g. 



4. CaCU 0.2 g. 



5. KH0PO4 1-5 g. 



6. NH4NO3 1-5 g. 



7. FeCU trace 



8. Sand 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Make slightly alkaline by the addi- 

 tion of Na2C03. 



(3) Distribute in 100.0 cc. lots. 



(4) Dissolve 0.5% of one of the added 

 nutrients in (3). 



(5) Add 12.0 cc. of (4) to 50.0 g. of sand. 

 Sterilization: Not specified. 



Use: Cultivation of Actinomyces odor if er. 



Act. chromogenes, Act. albus I and //, 



Act. S. a. b. and c. 

 Added nutrients: The author added 0.5% 



of one of the following: urea, sulpho- 



carbamide, alanin, tyrosin, dicyan- 



diamide. 

 Variants: Munter gave the following 



variants : 



(a) Used 1.0 g. K2HPO4 instead of 1.5 

 K2HPO4, omitted the trace of FeCU, 

 dissolved the salts in 500.0 cc. instead 

 of 1000.0 cc. of water, neutralized by 

 the addition of CaCOa. Instead of 

 adding 1.5 g. NH4NO3, 0.025 g. nitro- 

 gen was added in the form of NH4NO} 

 to each 100.0 cc. of solution. One of the 

 following (1.0%) was added: sucrose, 

 mannitol, galactose, lactose, inulin, 

 glucose, arabinose, levulose, glycerol, 

 starch. The solution was added to 

 sand as before. 



(b) Omitted the CaCU, used 1.0 g. 

 K2HPO4 instead of 1.5 g. K2HPO4, 

 omitted the FeCU, usedonly 500.0 cc. 

 of water instead of 1000.0 cc, made 

 slightly alkaline by the addition of 

 NajCOa, and added 0.0353 g. of nitro- 

 gen in the form of NH4NO3 to each 

 100.0 cc. of solution. To each 

 100.0 cc, 0.8 g. of one of the following 

 was added: oxalic acid, acetic acid, 

 succinic acid, malic acid, tartaric 

 acid, citric acid, hippuric acid, uric 

 acid, humus acid and aspartic acid. 

 The solution was added to sand as 

 before. 



Reference: Munter (1913 p. 377). 



2518. Gowda's Ammonium Sulphate Sand 

 Medium 



Constituents: 



1. Water (conductivity) 1100.0 cc. 



2. (NH4)2S04 10 g. 



3. K2HPO4 10 g. 



4. NaCl 2.0 g. 



