CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



827 



5. MgS04 0.5 g. 



6. FesCSOOs trace 



7. MgCOa 5.0 g. 



8. Sand 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1000.0 cc. 

 conductivity water. 



(2) Dissolve 7 in 100.0 cc. conductivity 

 water. 



(3) Mix (1) and (2). 



(4) Wash sand (Method not given). 



(5) Mix solution (3) with sand (amount 

 not given) and place in Petri dishes. 



Sterilization : Method not given. 



Use: To study nitrification by nitrate and 

 nitrite forms. The author reported that 

 nitrites were found in 10 days and nitrate 

 after 14 days. 



Reference: Gowda (1924 p. 259). 



2519. Wichers' Ammonium Sulphate Sand 



Medium 

 Constituents : 



1. Mineral Solution (nitrogen free). 



2. Sand. 



3. (NH4)2S04. 



4. MgCOs. 

 Preparation : 



(1) Distribute nitrogen free mineral solu- 

 tion (composition not given) into 

 200.0 cc. Erlenmeyer flasks in 50.0 cc. 

 lots. 



(2) Add 24.0 g. sand to each flask. 



(3) Add 10.0 cc. of a sterile 1.0% 

 (NH4)2S04 solution and a knifepoint 

 of sterile MgCOa to each sterile flask 

 of (2). 



Sterilization: Sterilize (2) in the autoclave. 

 Method of sterilization of (NH4)2S04 

 solution not given. 



Use: To study nitrification by nitrifying 

 organisms. The author reported that 

 nitrification was furthered in the presence 

 of permutite. More permutite may be 

 used. 



Reference: Wichers (1920 p. 1). 



2520. Malenkovic's Basal Nitrate Sand 

 Medium 

 Constituents: 



1- Water lOO.o cc. 



2-KN03 0.15g. 



3. (NH4)2HP04 015e 



4. K2HPO4 01 g 



5- MgS04 o.05g. 



6. Sand 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Prepare 10.0% solutions of one of the 

 soluble added nutrients in (1) and 

 saturate pure Quartz sand (sea sand) 

 with the solution. To those added 

 nutrients that are solid, add (1) 

 until they are saturated. 



Sterilization: Not specified. 



Use: Cultivation of Coniophora cerebella 



(Corticium putaneum). The author re- 

 ported that all materials from which 



dextrose is derived were good sources of 



carbon. 

 Added nutrients: The author used one of 



the following materials: 



glucose 



levulose 



d-mannose 



d-galactose 



maltose 



sucrose 



lactose 



dextrin 



starch 



mannitol 



dulcitol 



xylan 



arabinose 



tannic acid 



peptone 



rhamnose 



hog fat 



The author saturated one of the following 

 with the basic solution: 

 filter paper 

 cotton 



cotton (soluble in KOH) 

 sulfite pulp (unbleached) 

 cellulose from pine 

 cellulose from beechwood 

 Reference: Malenkovic (1906 p. 412). 



2521. Stigell's Nitrate Sand Medium 

 Constituents : 



1- Water 992.5 cc. 



2. Ca(N03)2 40 g 



3. KNO3 lOg 



4. MgS04 + 7H20 10 g 



5. K2HPO4 lOg' 



6. FeoCls 



7. Quartz sand 



