828 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Pour (1) over heated quartz sand 

 (6 liters of (1) on 60 kgm. of sand). 



Sterilization: Not specified. 



Use: To study influence of bacteria on 

 plants. The sand was inoculated with 

 bacteria and seeds were planted. The 

 following bacteria were used Bacillus 

 megatherium, Bacillus mesentericus fus- 

 cus, Proteus vulgaris, Bacillus pyocyaneus. 

 Bacillus subtilis, Bad. coli commune. 

 Bacillus prodigiosus, Bacillus butyricus. 

 The author reported that bacteria had 

 little influence on germination ability. 



Reference: Stigell (1909 p. 733). 



2522. Kriiger and Schneidewind's Basal 

 Glucose Sand Medium 



Heat sand to glowing, boil with HCl, 

 heat to glowing and then saturate with 

 medium 158. 

 Reference: Heinze (1906 p. 647). 



2523. Ashby's Basal Mannitol Sand Medium 

 (Reed and Williams) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Mannitol 12.0 g. 



3. KH2PO4 0.2 g. 



4. MgS04 0.2 g. 



5. NaCl 0.2 g. 



6. CaS04 0.1 g. 



7. CaCOa 5.0 g. 



8. Sand 

 Preparation : 



(1) Wash pure sea sand and burn. 



(2) Distribute 15.0 g. of (1) in liter Erlen- 

 meyer flasks. 



(3) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(4) Add 100.0 cc. of (3) to each flask of 

 (2) containing sand. 



(5) Add from 250 to 2000 parts per 

 million of one of the sterile added 

 nutrients to each flask. 



Sterilization: Sterilize (4) at 15 pounds 

 pressure for 15 minutes. Method of 

 sterilization of the added nutrients not 

 given. 



Use: To study nitrogen fixation. The 

 authors reported that the fixation of 

 nitrogen by Azotobacter was only slightly 

 influenced by any of the compounds 

 listed. 



Added nutrients: The author added one 



of the following: 



esculin 



vanillin 



daphnetin 



cumarin 



pyrocatechin 



heliotropin 



arbutin 



resorcin 



pyrogallol 



phloroglucin 



hydroquinone 



salicylic aldehyde 



oxalic acid 



quinic acid 



di-hydroxystearic acid 



rhamnose 



borneol 



caffeine 



betaine hydrochloride 



trimethylamine 



legumin 



alloxan 



cinnamic acid 



asparaginic acid 



hippuric acid 



creatine 



creatinine 



xanthine 



hypoxanthine 



urea 



formamide 



glycocoU 



allantoin 



guanidine carbonate 



nicotine 



picoline 



skatol 



piperidine hydrochloride 

 Reference: Reed and Williams (1915 



p. 168). 



2524. Munter's Basal Carbohydrate 

 Asparagin Sand Medium 



Constituents: 



1 Water 1000.0 cc. 



2. MgS04 0.5 g. 



3. NaCl 0.5 g. 



4. K2HPO4 1.5 g. 



5. CaCU 0.1 g. 



6. FeClj few drops 



7. Glucose 10.0 g. 



