CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



829 



8. Glycerol 5.0 g. 



9. Sand 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Distribute in 12.0 cc. lots. 



(3) Add 12.0 cc. of (2) and 0.1 g. of one 

 of the added nutrients to 50.0 g. sand. 



Sterilization: Method not given. 



Use: Cultivation of Actinomyces odorifer, 

 Act. chromogenes, Act. albus I and //, 

 Act. S. a, b and c. The author reported 

 that alanin and tyrosin were the best 

 nitrogen sources for the actinomycetes 

 studied. The remainder of the com- 

 pounds, however, were good sources for 

 some of the actinomycetes. 



Added nutrients: The author added one of 

 the following; urea, sulphocarbamide 

 alanin, tyrosin, dicyandiamide. 



Variants: The author substituted 2.0 g. 

 mannitol for 5.0 g. glycerol, used 2.0 g. 

 K2HPO4 instead of 1.5 g. KiHPOi in the 

 basal solution. Then he used 0.02 or 

 0.1 g. of nitrogen in the form of NH4XOS, 

 NH4CI or asparagin for added nutrients. 



Reference: Miinter (1913 p. 368, 377). 



2525. Heinze's Beerwort Sand Medium 



Constituents: 



1. Beerwort. 



2. Sand. 

 Preparation : 



(1) Heat sand to glowing. 



(2) Boil with HCl. 



(3) Reheat to glowing. 



(4) Saturate (3) with beerwort. 

 Sterilization: Not specified. 



Use: To study the nitrogen assimilation 

 of green algae, chlorella, chlorothecium 

 stichococcus. Heinze reported that nitro- 

 gen was not assimilated. 



Reference: Heinze (1906 p. 647). 



2526. Ej-iiger and Schneidewind's Extract 

 Broth Sand Medium (Heinze) 



Constituents: 



1. Water 1000.0 cc. 



2. Meat extract (0.5%) 5.0 g. 



3. Peptone (0.5%) 5.0 g. 



4. Glucose (0.5%,) 5.0 g. 



5. Sand 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Heat sand to glowing. 



(3) Boil (2) with HCI. 



(4) Reheat to glowing. 



(5) Saturate (4) with (1). 

 Sterilization: Xot specified. 



Use: To study the nitrogen assimilation by 

 green algae, chlorella, chlorothecium 

 stichococcus. Heinze reported that ni- 

 trogen was not as.similated. 



Reference: Heinze (1906 p. 647). 



2527. Stigell's Bouillon Sand Medium 



Constituents: 



1. Water 97.0 cc. 



2. Bouillon 3.0 cc. 



3. Quartz sand 300.0 g. 



Preparation : 



(1) Prepare bouillon. 



(2) Mix 300.0 g. of quartz sand, 3.0 cc. (1) 

 and 97.0 cc. of water. 



(3) Place in Petri dishes. 

 Sterilization : Method not given. 



Use: To study influence of bacteria on rate 

 of evaporation using Bac. subtilis, Pro- 

 teus vulgaris, Bac. coli communis, and 

 Bac. mesentericus fuscus. The author 

 reported that evaporation was inhibited 

 by the growth of bacteria. Amount of 

 evaporation determined by loss of weight 

 of cultures. 



Reference: Stigell (1908 p. 60). 



2528. Heinze's Loam Sand Medium 



Constituents : 



1. Distilled water. 



2. Sand. 



3. Loess loam. 

 Preparation: 



(1) Add 35.0% sand to soft Loess loam 

 soil rich in humus. 



(2) Saturate with sterile distilled water. 

 Sterilization: Not specified. 



Use: To study nitrogen assimilation by 

 green algae, chlorella, chlorothecium, 

 stichococcus. Heinze reported that ni- 

 trogen was not assimilated. 



Reference: Heinze (1906 p. 647). 



2529. Mortensen's "Starre" Media 



Constituents: 



1. Water 1000.0 cc. 



2. Sucrose (10.0%,) 100.0 g. 



3. (NH4)2S04 (0.5%) 5.0 g. 



4. Cobalt salts 



5. "Starre" materials 



