CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



831 



2533. Rossler's Iron Sulphate Brick 

 Medium 



Constituents: 



1. Water. 



2. Iron vitrei (hydrous ferrous sulphate). 



3. Brick. 

 Preparation : 



(1) Prepare a solution of iron vitrol in 

 water. 



(2) Place a sterile brick bat in (1). 

 Sterilization: Sterilize the brick bat by 



heating to glowing. Method of steriliza- 

 tion of (1) not given. 



Use: Cultivation of Crenothrix polypora. 



Reference: Rossler (1895 p. 189). 



2534. 



Mortensen's Basal Nitrate 

 "Starre" Medium 



Same as medium 329, but the solution is 

 added to one of the listed pulverized ma- 

 terials. The solid materials are placed in a 

 Petri dish, and the solution added until 

 they are about one-half immersed. The 

 materials used were the same as in medium 

 2529. 



2535. Greaves' Mannitol Soil Medium 



Constituents: 



1. Distilled water. 



2. Soil. 



3. Mannitol. 

 Preparation : 



(1) Sterilize tumblers covered with Petri 

 dishes. 



(2) Add 100.0 g. of soil and 1.5 g. man- 

 nitol. 



(3) Mix thoroly with a sterile spatula. 



(4) Add enough sterile distilled water to 

 make the moisture content about 

 18.0%. 



Sterilization: Methods not given. 



Use: To study nitrogen assimilation by 

 soil forms. Nitrogen was determined by 

 Lipman and Sharp's (Centr. f. Bakt., II 

 Abt., 35: 648, 1913) modified Kjeldahl's 

 method. The author reported that this 

 medium was used to determine the nitro- 

 gen assimilation ability of different soils 

 and 100.0 g. of the soils in question were 

 used in (2). 



Reference: Greaves (1914 p. 454). 



2536. Vierling's Cellulose Soil Medium 



Constituents : 



1. Soil. 



2. Filter paper. 



Preparation: (1) Place a piece of filter 

 paper between garden soil in a Petri dish. 



Sterilization: Method of sterilization of (1) 

 not given. 



Use: Decomposition of cellulose. After 

 inoculation place the medium under a 

 glass bell jar. The author reported that 

 filter paper showed no signs of being 

 attacked. Growth occurred, however. 



Reference: Vierling (1920 p. 206). 



2537. Mortensen's Ammonium Sulphate 



"Starre" Medium 



Constituents : 



1. Water 1000.0 cc. 



2. Sucrose (10.0%) 100.0 g. 



3. (NH4)2S04 (0.5%) 5.0 g. 



4. Cobalt salts 



5. "Starre" materials 



Preparation: Same as for medium 2534, 

 but using a solution of 2, 3 and 4 in 1 

 instead of the basal solution. 



Sterilization: Not specified. 



Use : See medium 329. 



Reference: Mortensen (1909 p. 532). 



2538. Zikes' Glucose Peptone Soil 

 Medium 



Constituents: 



1. Water. 



2. Soil. 



3. Glucose. 



4. Peptone. 

 Preparation : 



(1) Place soil in Erlenmeyer flasks to a 

 depth of 1.0 cm. 



(2) Pour water on one side and a 1.0% 

 solution of glucose and peptone in the 

 other side of the flask until the soil 

 is dampened. 



Sterilization: Sterilize (Method not given). 



Use: Cultivation of Apiculatus yeast, 

 Torula alba, Torula Molischiana Myco- 

 derma cerevisiae, Blastoderma salmoni- 

 color. Author reported that due to small 

 glucose content the yeast produced 

 spores on this medium. 



Reference: Zikes (1911 p. 147). 



