832 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2539. Mortensen's Peptone "Starre" 

 Medium 



Same as medium 560, but containing 

 "starre" materials as given in medium 



2529. 



2540. Lipman's Dried Blood Soil Medium 

 Constituents : 



1. Distilled water 18.0 cc. 



2. Soil 100.0 g. 



3. Dried blood 2.0 g. 



4. NaCl 0.0 to 2.0% 



Preparation : 



(1) Add 2.0 g. of dried blood to each of a 

 series of glass tumblers containing 

 100.0 g. of soil. 



(2) To each tumbler add varying amounts 

 of NaCl, increasing the salt concen- 

 tration by 0.2% each time. 



(3) Mix thoroly with a sterile dry spatula. 



(4) Add 18.0 g. of sterile distilled water 

 to each flask and mix thoroly. 



(5) Cover the tumblers with clean Petri 

 dish covers. 



Sterilization: Not specified. 



Use: To study ammonification by soil bac- 

 teria. The author reported that ammo- 

 nification was inhibited by various 

 amounts of NaCl, Na2S04 and NaaCOs. 



Variants: The author used Na2S04, or 

 NasCOs instead of NaCl. 



Reference: Lipman (1911-12 p. 58). 



2541. Greaves' Dried Blood Soil Medium 

 Constituents : 



1. Water. 



2. Soil. 



3. Dried blood. 

 Preparation : 



(1) Sterilize tumblers covered with Petri 

 dishes. 



(2) Weigh 100.0 g. of soil and 2.0 g. dried 

 blood into each tumbler. 



(3) Mix thoroly with a sterile spatula. 



(4) Add sterile distilled water to make 

 the moisture content about 18.0%. 



Sterilization: Method not given. 

 Use: To study denitrification. 

 Reference: Greaves (1914 p. 449). 



2542. Schonbrunn's Horn Meal Soil 

 Medium 

 Constituents : 



1. Distilled water 600.0 cc. 



2- Soil 3000.0 g. 



3. Horn meal 50.0 g. 



Preparation : 



(1) Mix thoroly 3000.0 g. soil with 50.0 g. 

 horn meal. 



(2) Place in glass beakers 25.0 cm. high 

 and 15 cm. wide. 



(3) Add 600.0 cc. distilled water. 



(4) Cover the beakers with parchment 

 paper. 



Sterilization: Not specified. 

 Use: To study nitrification. 

 Reference: Schonbrunn (1922 p. 549). 



2543. Lohnis and Green's Phosphate Meal 

 Medium 



Constituents : 



1. Water (tap) lOOO.O cc. 



2. K0HPO4 (0.05%) 0.5 g. 



3. Soil 



4. Flesh meal 

 Preparation : > 



(1) Dissolve 0.05% K2HPO4 in tap water. 



(2) Mix 0.4 or 2.0 g. flesh meal with 

 50.0 cc. of (1). 



(3) Add (2) to 100.0 g. soil (dry weight). 

 Sterilization: Not specified. 



Use: To study ammonification by soil 



forms. 

 Variants: The authors used 0.2 or 1.0 g. 



blood or horn meal instead of flesh meal. 

 Reference: Lohnis and Green (1913 p. 536). 



